Enzyme-linked immunosorbent assays using recombinant envelope protein expressed in COS-1 and Drosophila S2 cells for detection of West Nile virus immunoglobulin M in serum or cerebrospinal fluid

Clinical and Diagnostic Laboratory Immunology
A Scott MuerhoffSuresh M Desai

Abstract

Humans infected with West Nile virus (WNV) develop immunoglobulin M (IgM) antibodies soon after infection. The microtiter-based assays for WNV IgM antibody detection used by most state public health and reference laboratories utilize WNV antigen isolated from infected Vero cells or recombinant envelope protein produced in COS-1 cells. Recombinant antigen produced in COS-1 cells was used to develop a WNV IgM capture enzyme immunoassay (EIA). A supplementary EIA using WNV envelope protein expressed in Drosophila melanogaster S2 cells was also developed. Both assays detected WNV IgM in the sera of experimentally infected rhesus monkeys within approximately 10 days postinfection. Human sera previously tested for WNV IgM at a state public health laboratory (SPHL) were evaluated using both EIAs. Among the sera from 20 individuals with laboratory-confirmed WNV infection (i.e., IgM-positive cerebrospinal fluid [CSF]) that were categorized as equivocal for WNV IgM at the SPHL, 19 were IgM positive and one was negative by the new EIAs. Of the 19 IgM-positive patients, 15 were diagnosed with meningitis or encephalitis; the IgM-negative patient was not diagnosed with neurological disease. There was 100% agreement between the EIAs for the d...Continue Reading

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Citations

May 10, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Harry E PrinceMichael P Busch
Jan 12, 2007·Journal of Virological Methods·Sebastien HerrmannRobert S Marks
Mar 5, 2014·Transboundary and Emerging Diseases·K BerxholiM H Groschup
Apr 12, 2012·World Journal of Virology·Miguel A Martín-Acebes, Juan-Carlos Saiz
Oct 19, 2004·Clinical Microbiology Reviews·Anthony W SolomonDavid C W Mabey
Oct 24, 2007·Analytical Chemistry·Rodica E IonescuRobert S Marks

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