Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread

Annals of Clinical Microbiology and Antimicrobials
Rupak ThapaPravin Dudhagara

Abstract

Silver nanoparticles (AgNPs) are believed to be emerging tool against various infectious diseases including multi-drug resistant (MDR) bacteria. In the present study, in vitro synthesis of AgNPs was optimized using 1:50 ratio of macerozyme (25 μg/μl) and 1 mM AgNO3 incubated at 80 °C for 8 h. AgNPs were characterized by UV-Visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD). Characterization studies suggest the synthesis of elliptical, stable and crystalline AgNPs with an average size of 38.26 ± 0.4 nm calculated using TEM. The XRD pattern revealed the face-centered-cubic (fcc) form of metallic silver. Good shape integrity and dispersion of AgNPs after 1 year of incubation confirmed their stability. AgNPs were exibited the antimicrobial property against ten pathogenic bacteria, three molds and one yeast. The AgNPs also revealed remarkable antimicrobial activity against three MDR strains i.e. Extended spectrum beta-lactamase positive Escherichia coli, Staphylococcus aureus (MRSA) and Teicoplanin resistant Streptococcus Pneumoniae. The AgNPs coated surgical threads (suture) were revealed the rem...Continue Reading

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Citations

Aug 7, 2019·International Journal of Molecular Sciences·María Vallet-RegíIsabel Izquierdo-Barba
Jul 18, 2018·Frontiers in Microbiology·Pedro V BaptistaAlexandra R Fernandes
Mar 7, 2019·Current Drug Discovery Technologies·Chew-Li MooKok-Song Lai
Mar 12, 2021·Journal of Drug Targeting·Khatereh KhorsandiSimab Kanwal
Aug 9, 2020·Journal of Hazardous Materials·Mahmoud NasrollahzadehRajender S Varma
Jul 28, 2020·Heliyon·Sunday Adewale AkinteluAbel Kolawole Oyebamiji
Aug 28, 2021·Antibiotics·Marcela Nowak, Wioletta Barańska-Rybak

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