Enzyme-Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One-Pot Cascade Reactions

Angewandte Chemie
Zhiyong SunChangzhu Wu

Abstract

Despite the rapid development of Pickering interfacial catalysis (PIC) at liquid-liquid interfaces with chemocatalysts, the use of unstable biocatalysts at emulsion interfaces remains a technical challenge. Herein, we present a Pickering interfacial biocatalysis (PIB) platform based on robust and recyclable enzyme-polymer conjugates that act as both catalytic sites and stabilizers at the interface of Pickering emulsions. The conjugates were prepared by growing poly(N-isopropylacrylamide) on a fragile enzyme, benzaldehyde lyase, under physiological conditions. The mild in situ conjugation process preserved the enzyme structure, and the conjugates were used to emulsify a water-organic two-phase system into a stable Pickering emulsion, leading to a significantly larger interfacial area and a 270-fold improvement in catalytic performance as compared to the unemulsified two-phase system. The PIB system could be reused multiple times. Conjugates of other enzymes were also fabricated and applied for cascade reactions.

References

Feb 19, 1998·Journal of Biomedical Materials Research·Z DingA S Hoffman
Apr 28, 2001·Biophysical Journal·J T Vivian, P R Callis
Nov 11, 2003·Biomacromolecules·Dirk SchmaljohannCarsten Werner
May 19, 2011·ChemSusChem·Pierrick GaudinFrançois Jérôme
Jun 21, 2011·Chemical Communications : Chem Comm·Patrick van RijnAlexander Böker
Nov 11, 2011·Advanced Materials·Changzhu WuDayang Wang
Jun 26, 2012·Chemistry : a European Journal·Panchao YinTianbo Liu
Sep 22, 2012·Chemistry : a European Journal·Loïc LeclercqVéronique Nardello-Rataj
Oct 2, 2012·Angewandte Chemie·Zhipeng WangJan C M van Hest
Dec 6, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Patrick van RijnAlexander Böker
Jan 26, 2013·Advanced Synthesis & Catalysis·Tina GerhardsDörte Rother
Apr 20, 2013·Journal of the American Chemical Society·Qiang ZhangDavid M Haddleton
Mar 19, 2014·Journal of the American Chemical Society·Wen-Juan ZhouJean-Marc Clacens
May 7, 2014·Chemical Communications : Chem Comm·Xin HuangStephen Mann
Jun 7, 2014·Journal of the American Chemical Society·Xin HuangStephen Mann
Oct 23, 2014·Nature Communications·Daniel ThielJan Deska
Nov 18, 2014·Nature Materials·Isidro CoboSébastien Perrier
May 5, 2016·Angewandte Chemie·Xiaoman LiuStephen Mann
Mar 11, 2017·Nature Chemistry·Yan QiaoStephen Mann
Mar 30, 2017·Soft Matter·Himanshu CharanAlexander Böker
Apr 14, 2017·Journal of the American Chemical Society·Kingshuk DuttaS Thayumanavan
Oct 3, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Jinghui WangZhimin He
Dec 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Guojun LvBernard P Binks
Jan 11, 2018·APL Bioengineering·Federica RigoldiAlfonso Gautieri
Apr 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiafu ShiZhongyi Jiang

❮ Previous
Next ❯

Citations

Jan 31, 2019·Chembiochem : a European Journal of Chemical Biology·Lin LuQian Wang
May 7, 2020·Advanced Materials·Xiaoliang WangXin Huang
Oct 31, 2020·Advanced Materials·Lang MaLi Qiu
Jan 19, 2021·Chemical Communications : Chem Comm·Marko PavlovicLukas Zeininger
Jan 23, 2021·Angewandte Chemie·Zhiyong SunChangzhu Wu
Jul 2, 2021·Advanced Materials·Meng LiPeng Huang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.