PMID: 6172520Jan 1, 1981Paper

Enzyme potentiated radioimmunoassay (EPRIA): a sensitive third-generation test for the detection of hepatitis B surface antigen

Journal of Immunological Methods
H A FieldsJ E Maynard

Abstract

A sensitive, specific immunoassay for detection of hepatitis B surface antigen (HBsAg) is described. The assay combined enzyme-linked immunosorbent assay and solid-phase radioimmunoassay and is termed enzyme potentiated radioimmunoassay (EPRIA). HBsAg was quantitated by enzymatic conversion of L[14C]glutamic acid to 14CO2 and gamma-aminobutyric acid by glutamate decarboxylase (GDC) conjugated wih goat anti-HGs IgG. Conjugation of IgG and GDC was by a thiol-disulfide bond exchange reaction after reacting N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) with each reagent. A positive/negative ratio of 2.2 was established as significant by examination of 40 normal sera negative for HBsAg. This value was the mean cpm plus 3 standard deviations. By an identical statistical analysis of sensitivity, EPRIA was found to be approximately 100-fold more sensitive than Ausria II (Abbott Laboratories, North Chicago, IL).

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C C HarrisI C Hsu
Jan 1, 1979·Antibiotics and Chemotherapy·R F Jarvis
Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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