Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing

ACS Nano
Xiaolong YangFeng Li

Abstract

Herein, we report a DNA nanomachine, built from a DNA-functionalized gold nanoparticle (DNA-AuNP), which moves a DNA walker along a three-dimensional (3-D) DNA-AuNP track and executes the task of releasing payloads. The movement of the DNA walker is powered by a nicking endonuclease that cleaves specific DNA substrates on the track. During the movement, each DNA walker cleaves multiple substrates, resulting in the rapid release of payloads (predesigned DNA sequences and their conjugates). The 3-D DNA nanomachine is highly efficient due to the high local effective concentrations of all DNA components that have been co-conjugated on the same AuNP. Moreover, the activity of the 3-D DNA nanomachine can be controlled by introducing a protecting DNA probe that can hybridize to or dehybridize from the DNA walker in a target-specific manner. This property allows us to tailor the DNA nanomachine into a DNA nanosensor that is able to achieve rapid, isothermal, and homogeneous signal amplification for specific nucleic acids in both buffer and a complicated biomatrix.

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Citations

Sep 27, 2016·ACS Nano·Xing MaSamuel Sánchez
Oct 6, 2016·Journal of the American Chemical Society·Xiaolong YangFeng Li
Jan 13, 2017·Angewandte Chemie·Xiangmeng QuChunhai Fan
Dec 3, 2016·Nucleic Acids Research·Pai PengTao Li
Mar 7, 2017·Nature Communications·Hanyong PengX Chris Le
May 4, 2017·Chemical Communications : Chem Comm·Wei LiWei Jiang
Nov 23, 2017·Nanoscale·Linqiang PanCheng Zhang
Sep 23, 2018·Chemistry : a European Journal·Xiaoxi ZhongRongxing Zhou
Apr 17, 2019·Angewandte Chemie·Lei WangSamuel Sánchez
Dec 14, 2019·Chemical Communications : Chem Comm·Namin HuXia Chu
Feb 27, 2020·Chemical Communications : Chem Comm·Chang LiuXia Chu
Sep 23, 2020·Chemical Communications : Chem Comm·Xiaowen LiuQingwang Xue
Oct 31, 2018·Biosensors·Marta Maria Pereira da Silva Neves, Daniel Martín-Yerga
Dec 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dinggeng HeHung-Wing Li
Feb 22, 2018·Nanoscale·Scott G HarrounAlexis Vallée-Bélisle
May 8, 2019·Chemical Communications : Chem Comm·Xiaowen XuWei Jiang
Jul 28, 2020·Advanced Science·Yaqin HuYanfei Liu
Aug 14, 2019·Chemical Society Reviews·Wenjing WangJun-Jie Zhu

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