Enzyme sensing based on a controllable oxidation reaction

Biosensors & Bioelectronics
Ruilong ShengShi-Kang Wu

Abstract

We developed a new method for glutathione reductase (GR) enzyme sensing via a metal-controlled spontaneous oxidation reaction. A new complex HgL, composed of 3-benzothiazoliny-7-N,N-diethylaminocoumarin (L) and HgCl2, was used as an example for illustration. It was found that ligand L was released from complex HgL by a ligand exchange process in the presence of GSH, which was enzymatic reduced from GSSG in the presence of NADH. Subsequently, free ligand L was spontaneously oxidated to a laser dye (coumarin-6) with both fluorescence enhancement and color change. A good linear relationship between fluorescent intensity and GSH concentrations (in the range of 10-40 μM) elicits the potential use of HgL in GSH detection. Based on this result, complex HgL was applied to identify GR from other proteins/enzymes with good selectivity and sensitivity. HgL can also determine GR in the concentration range from 0.5 to 10 U/mL with a linear relationship. Furthermore, this novel sensing method was also expected to be a useful example for the design of other biosensing systems.

Citations

Feb 24, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Junbo LiZhiquan Pan
Sep 12, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoli WuGuang-Ming Bao
Sep 29, 2018·Journal of Agricultural and Food Chemistry·Qiao HuJi-Ting Hou

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