PMID: 9554847May 9, 1998Paper

Enzyme structure with two catalytic sites for double-sieve selection of substrate

Science
O NurekiShigeyuki Yokoyama

Abstract

High-fidelity transfers of genetic information in the central dogma can be achieved by a reaction called editing. The crystal structure of an enzyme with editing activity in translation is presented here at 2.5 angstroms resolution. The enzyme, isoleucyl-transfer RNA synthetase, activates not only the cognate substrate L-isoleucine but also the minimally distinct L-valine in the first, aminoacylation step. Then, in a second, "editing" step, the synthetase itself rapidly hydrolyzes only the valylated products. For this two-step substrate selection, a "double-sieve" mechanism has already been proposed. The present crystal structures of the synthetase in complexes with L-isoleucine and L-valine demonstrate that the first sieve is on the aminoacylation domain containing the Rossmann fold, whereas the second, editing sieve exists on a globular beta-barrel domain that protrudes from the aminoacylation domain.

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J J PeronaS Brunie
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Sep 25, 1987·Science·R M StarzykP Schimmel
Jan 1, 1994·Methods in Enzymology·N D Rawlings, A J Barrett
Nov 7, 1996·Nature·L LinP Schimmel
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Oct 3, 2002·Biochemical and Biophysical Research Communications·Masaki GoudaKazuya Nishikawa
Mar 29, 2003·Journal of Molecular Biology·Chun-Mei ZhangYa-Ming Hou
Apr 15, 2003·Journal of Molecular Biology·Timothy L BullockJohn J Perona
Feb 24, 2000·Biochimica Et Biophysica Acta·J M BullardL L Spremulli
Sep 24, 1999·Biochimie·S A MartinisC Florentz
Feb 19, 2000·Current Opinion in Structural Biology·S BlanquetE Schmitt
Jun 20, 2001·Bioorganic & Medicinal Chemistry Letters·T K Nomanbhoy, P Schimmel
Oct 9, 2001·Trends in Biochemical Sciences·L Ribas de Pouplana, P Schimmel
May 10, 2006·Biochemistry·Joshua S Weinger, Scott A Strobel
Mar 2, 2006·Journal of the American Chemical Society·Deqiang ZhangJ Andrew McCammon
Sep 24, 2009·Journal of the American Chemical Society·Duy P NguyenJason W Chin
Feb 9, 2010·Journal of the American Chemical Society·Yohsuke HagiwaraMasaru Tateno
Sep 13, 2005·Nature Structural & Molecular Biology·Ryuya Fukunaga, Shigeyuki Yokoyama
Oct 24, 2002·European Journal of Biochemistry·Ita Gruic-SovuljIvana Weygand-Durasevic
Sep 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kenji OkiShigeyuki Yokoyama
Oct 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Benfang RuanDieter Söll
Nov 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michal T BonieckiSusan A Martinis
Dec 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Srujana S YadavalliMichael Ibba
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jiqiang Ling, Dieter Söll
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tanweer HussainRajan Sankaranarayanan
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dino Moras
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li LiMichal T Boniecki
Feb 16, 2012·The Journal of Biological Chemistry·Michal T Boniecki, Susan A Martinis
May 26, 2010·The Journal of Biological Chemistry·Morana DulicIta Gruic-Sovulj
Jul 20, 2011·The Journal of Biological Chemistry·Byung Ran SoKarin Musier-Forsyth
Oct 4, 2011·The Journal of Biological Chemistry·Elvira Olmedo-VerdIgnacio Luque
Oct 29, 2008·The Journal of Biological Chemistry·Xiao-Long ZhouEn-Duo Wang
Jul 22, 2008·The Journal of Biological Chemistry·Paul Schimmel
Nov 8, 2001·Journal of Biomolecular Structure & Dynamics·A SchlosserM Sprinzl
Jul 29, 2009·Antioxidants & Redox Signaling·Merridee A WoutersNaomi L Haworth
May 29, 2001·EMBO Reports·M Ibba, D Söll
Sep 16, 1998·The EMBO Journal·J CavarelliD Moras
May 29, 2002·The EMBO Journal·Kate J NewberryJohn J Perona

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