Enzyme systems for biodegradation of polychlorinated dibenzo-p-dioxins

Applied Microbiology and Biotechnology
Toshiyuki Sakaki, Eiji Munetsuna

Abstract

The angular dioxygenase, cytochrome P450, lignin peroxidase, and dehalogenase are known as dioxin-metabolizing enzymes. All of these enzymes have metal ions in their active centers, and the enzyme systems except for peroxidase have each distinct electron transport chain. Although the enzymatic properties of the angular dioxygenase, lignin peroxidase, and cytochrome P450 have been studied well, the information about dehalogenase is much less than other enzyme systems due to its instability under the aerobic conditions. However, this enzyme system appears to be quite promising from the viewpoint of practical use for bioremediation, because dehalogenases are capable of degradation of polychlorinated dibenzo-p-dioxins (PCDDs) with more than four chlorine substituents, whereas the other three enzyme systems prefer low-chlorinated PCDDs. On the other hand, protein engineering of angular dioxygenase, lignin peroxidase, and cytochrome P450 based on their tertiary structures has great potential to generate highly efficient dioxin-metabolizing enzymes. Actually, we successfully generated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-metabolizing enzyme by site-directed mutagenesis of cytochrome P450. We hope that recombinant microorganisms ...Continue Reading

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Citations

Apr 12, 2012·Applied and Environmental Microbiology·Erica M HartmannRolf U Halden
Jun 13, 2012·Biological & Pharmaceutical Bulletin·Toshiyuki Sakaki
Aug 16, 2014·International Journal of Molecular Sciences·Hideyuki InuiToshiyuki Sakaki
Jan 30, 2013·Trends in Biochemical Sciences·Andrew W MunroKirsty J McLean
Oct 5, 2011·Biotechnology Advances·Owen P Ward
Apr 17, 2013·Biotechnology and Applied Biochemistry·Toshiyuki SakakiShinichi Ikushiro
Oct 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weihua WangPing Li
Oct 30, 2020·Journal of Hazardous Materials·Bao-Anh Thi NguyenChieh-Chen Huang

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