Enzyme transient state kinetics in crystal and solution from the perspective of a time-resolved crystallographer

Structural Dynamics
Marius Schmidt, Dilano K Saldin

Abstract

With recent technological advances at synchrotrons [Graber et al., J. Synchrotron Radiat. 18, 658-670 (2011)], it is feasible to rapidly collect time-resolved crystallographic data at multiple temperature settings [Schmidt et al., Acta Crystallogr. D 69, 2534-2542 (2013)], from which barriers of activation can be extracted. With the advent of fourth generation X-ray sources, new opportunities emerge to investigate structure and dynamics of biological macromolecules in real time [M. Schmidt, Adv. Condens. Matter Phys. 2013, 1-10] in crystals and potentially from single molecules in random orientation in solution [Poon et al., Adv. Condens. Matter Phys. 2013, 750371]. Kinetic data from time-resolved experiments on short time-scales must be interpreted in terms of chemical kinetics [Steinfeld et al., Chemical Kinetics and Dynamics, 2nd ed. (Prentience Hall, 1985)] and tied to existing time-resolved experiments on longer time-scales [Schmidt et al., Acta Crystallogr. D 69, 2534-2542 (2013); Jung et al., Nat. Chem. 5, 212-220 (2013)]. With this article, we will review and outline steps that are required to routinely determine the energetics of reactions in biomolecules in crystal and solution with newest X-ray sources. In eight sect...Continue Reading

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Citations

Oct 31, 2019·Wiley Interdisciplinary Reviews. Computational Molecular Science·David S Cerutti, David A Case
Mar 6, 2019·Nature Communications·Katherine A NiessenAndrea G Markelz
Jun 2, 2015·Soft Matter·S Khodadadi, A P Sokolov
Oct 14, 2020·Current Opinion in Structural Biology·Allen M Orville

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Methods Mentioned

BETA
nuclear magnetic resonance
X-ray
nuclear

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