Enzyme-triggered in situ formation of Ag nanoparticles with oxidase-mimicking activity for amplified detection of alkaline phosphatase activity

The Analyst
Hongwei SongXiangheng Niu

Abstract

Given that alkaline phosphatase (ALP) is an important biomarker for many diseases, monitoring of its activity turns to be of great significance for related disease diagnosis and treatment. Herein, we report a new colorimetric assay based on the enzyme-triggered in situ formation of Ag nanoparticles (NPs) with high oxidase-mimicking activity for ALP activity detection. ALP first hydrolyzes the ascorbic acid phosphate (AAP) substrate to produce ascorbic acid (AA); the produced AA with strong reducing capacity then transforms Ag+ into Ag NPs; compared with the Ag+ precursor, the in situ formed Ag NPs have much higher oxidase-like activity to catalyze the 3,3',5,5'-tetramethylbenzidine (TMB) color reaction mediated by dissolved O2 at neutral pH. On the basis of this principle, amplified colorimetric detection of ALP activity with a linear scope of 0.15-5 U L-1 and a limit of detection down to 0.037 U L-1 was realized. In addition, our assay exhibited specific response toward ALP against other biological enzymes and species. Accurate and reliable determination of ALP activity in human plasma was also demonstrated by our assay, suggesting its great potential as a facile and efficient tool for monitoring of ALP activity in clinical pr...Continue Reading

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Citations

Sep 10, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hongwei SongXiangheng Niu
Apr 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wei Feng, Yu Chen
Aug 14, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jiantao GuoTingting Cui
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing WangYin-Xian Peng
Jun 5, 2021·Journal of Biomedical Nanotechnology·Heping WangXue Xue
Dec 3, 2021·The Journal of Physical Chemistry Letters·Xingyu WangShuiqin Zhou

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays

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