PMID: 6403622Mar 11, 1983Paper

Enzyme/anti-enzyme monoclonal antibody soluble immune complexes (EMAC): their use in quantitative immunoenzymatic assays

Journal of Immunological Methods
T TernynckS Avrameas

Abstract

Enzyme/anti-enzyme antibody soluble immune complexes were prepared with monoclonal mouse antibodies (MA) which were directed against peroxidase (PO) and beta-galactosidase (GAL). These enzyme monoclonal antibody complexes (EMAC) functioned as markers to quantify mouse antibodies using an enzyme immunoassay which incorporated an anti-mouse Ig as the bridge between the EMAC and the specific antibody bound to an antigen immobilized on a polystyrene plate. EMAC prepared with PO (PO-MAC) or with GAL (GAL-MAC) were both effective in quantifying polyclonal as well as monoclonal mouse antibodies, and gave sensitivity equal or superior to that obtained with covalent enzyme/anti-mouse Ig conjugates. The smaller amount of antibodies detected with EMAC depended on the affinity of both the antibody tested and the monoclonal antibody used to prepare EMAC. This method is an improvement on the 'antibody bridge' method, because EMAC can be prepared easily by simply mixing the enzyme and the MA at the appropriate amounts 2 h prior to use. In addition, EMAC can be prepared using crude preparations of enzyme and unpurified ascitic fluids containing the MA, thus decreasing the cost of the test considerably.

References

Sep 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T E MasonR B Dreskin
Jan 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C A ClarkF J Primus
Jun 24, 1971·FEBS Letters·B K. Van Weemen, A H.W.M. Schuurs

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Citations

Feb 11, 1987·Journal of Immunological Methods·L KarawajewM Gaestel
Jun 24, 1992·Journal of Immunological Methods·T Porstmann, S T Kiessig
Jun 24, 1992·Journal of Immunological Methods·S Avrameas
Jan 1, 1987·Acta histochemica·P DráberV Viklický
Aug 10, 2000·Journal of Immunoassay·J E Butler
Jan 1, 1990·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M Kasper, P Stosiek
Aug 10, 2000·Journal of Immunoassay·V Kumar

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