PMID: 698245Sep 28, 1978Paper

Enzymes catalyzing the hydrolysis of long-chain monoacyglycerols in rat adipose tissue

Biochimica Et Biophysica Acta
H TornqvistP Belfrage

Abstract

Acetone-ether preparations of epididymal fat pads from fasted or fed rats contained two enzymes catalyzing the hydrolysis of long-chain monoacylglycerols. The enzymes were identified as monoacylglycerol lipase (Tornqvist, H. and Belfrage, P., (1976) J. Biol Chem. 251, 813--819) and lipoprotein lipase by their apparent pI values after electrofocusing in non-ionic detergent, selective inhibition properties, substrate specificity and positional specificity. It was estimated that monoacylglycerol lipase accounted for about 90% of the total monoacylglycerol-hydrolyzing activity in acetone-ether preparations from fasted and 70% from fed rats. Its enzyme activity did not change with the nutritional state in contrast to that of lipoprotein lipase. The latter enzyme hydrolyzed 2-monoacylglycerols at a much lower rate than the 1(3)-isomers. Monoacylglycerol lipase was located almost entirely in the adipocytes, thus most of the enzyme activity towards monoacylglycerols in the adipose tissue was found in this site. Fractionated sucrose homogenates of rat epididymal fat pads also contained a third enzyme with monoacylglycerol-hydrolyzing activity, identified as hormone-sensitive lipase by its pI, selective inhibition properties and substrat...Continue Reading

References

Apr 22, 1976·Biochimica Et Biophysica Acta·P Nilsson-EhleM C Schotz
May 1, 1986·Stroke; a Journal of Cerebral Circulation·M A RossV Dunn
Nov 18, 1974·Biochimica Et Biophysica Acta·P Arner, J Ostman
Aug 1, 1974·Lipids·P Nilsson-EhleM C Schotz
Apr 22, 1970·Biochimica Et Biophysica Acta·C J Fielding
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Citations

Sep 1, 1991·Brain Research Bulletin·R O Scow, E J Blanchette-Mackie
Aug 23, 2007·Chemistry & Biodiversity·Susanna M Saario, Jarmo T Laitinen
Jul 1, 1987·Cell Biochemistry and Function·A Verine, J Boyer
Jun 16, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A Girousse, D Langin
Sep 8, 2017·Diabetes, Obesity & Metabolism·Pegah PoursharifiMarc Prentki
Feb 12, 1986·Biochimica Et Biophysica Acta·C VannierG Ailhaud
Aug 15, 1984·Biochimica Et Biophysica Acta·S K ComptonP Hamosh

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