Enzymes II of the phosphotransferase system do not catalyze sugar transport in the absence of phosphorylation.

Journal of Bacteriology
P W Postma, J B Stock

Abstract

In Salmonella typhimurium, glucose, mannose, and fructose are normally transported and phosphorylated by the phosphoenolpyruvate:sugar phosphotransferase system. We have investigated the transport of these sugars and their non-metabolizable analogs in mutant strains lacking the phospho-carrier proteins of the phosphoenolpyruvate:sugar phosphotransferase system, the enzymes I and HPr, to determine whether the sugar-specific, membrane-bound components of the phosphonenolpyruvate: sugar phosphotransferase system, the enzymes II, can catalyze the uptake of these sugars in the absence of phosphorylation. This process does not occur. We have also isolated mutant strains which lack enzyme I and HPr, but have regained the ability to grow on mannose or fructose. These mutants contained elevated levels of mannokinase (fructokinase). In addition, growth on mannose required constitutive synthesis of the galactose permease. When strains were constructed which lacked the galactose permease, they were unable to grow even on high concentrations of mannose, although elevated levels of mannokinase (fructokinase) were present. These results substantiate the conclusion that the enzymes II of the phosphoenolpyruvate:sugar phosphotransferase system ...Continue Reading

References

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Citations

Jan 1, 2015·Applied Microbiology and Biotechnology·Masato IkedaSeiki Takeno
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·H L KornbergA A Sproul
Jan 31, 2003·The Journal of Biological Chemistry·Hans L Kornberg
Mar 1, 1982·European Journal of Biochemistry·P W PostmaR van Ree
Sep 1, 1983·Microbiological Reviews·K E Sanderson, J R Roth
Dec 1, 1986·Journal of Bacteriology·P W PostmaP Koolwijk
Sep 1, 1985·Microbiological Reviews·P W Postma, J W Lengeler
Jun 1, 1996·Applied and Environmental Microbiology·R LauretM Zagorec
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson

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