Enzymes in a golden cage.

Chemical Science
Yael Baruch-Shpigler, David Avnir

Abstract

We describe a general method for the entrapment of enzymes within bulk metallic gold. This is a new approach for the immobilization of enzymes on metals, which is commonly carried out by 2D adsorption or covalent biding, that is, the enzyme is in contact with the metal at a specific contact zone of the enzyme, while most of the rest of it remains exposed to the environment. The 3D metallic encaging of the enzymes is quite different: the enzyme is in contact with the metallic cage walls all around it and is well protected inside. The porous nature of the metallic matrix enables substrate molecules to diffuse inside, reach the active site, and let product molecules diffuse out. The generality of the approach was proven by the successful entrapment of five enzymes representing different classes and different bio- and medical applications: l-asparaginase (Asp), collagenase, horseradish peroxidase (HRP), laccase and glucose oxidase (GOx). GOx-gold conjugates have been of particular interest in the literature. The main challenge we had to solve was how to keep the enzyme active in the process of gold-synthesis from its cation - this required careful tailoring of reaction conditions, which are detailed in the paper. The gold entrapped...Continue Reading

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Citations

May 26, 2021·Scientific Reports·Yael Baruch-Shpigler, David Avnir

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BETA
biosensing
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