Enzymes in low water systems

Critical Reviews in Biochemistry and Molecular Biology
M Tuena de Gómez-Puyou, A Gómez-Puyou

Abstract

Water is fundamental for enzyme action and for formation of the three-dimensional structure of proteins. Hence, it may be assumed that studies on the interplay between water and enzymes can yield insight into enzyme function and formation. This has proven correct, because the numerous studies that have been made on the behavior of water-soluble and membrane enzymes in systems with a low water content (reverse micelles or enzymes suspended in nonpolar organic solvents) have revealed properties of enzymes that are not easily appreciated in aqueous solutions. In the low water systems, it has been possible to probe the relation between solvent and enzyme kinetics, as well as some of the factors that affect enzyme thermostability and catalysis. Furthermore, the studies show that low water environments can be used to stabilize conformers that exhibit unsuspected catalytic properties, as well as intermediates of enzyme function and formation that in aqueous media have relatively short life-times. The structure of enzymes in these unnatural conditions is actively being explored.

References

Mar 14, 1978·Biochimica Et Biophysica Acta·P DouzouF Franks
Dec 15, 1992·The Biochemical Journal·M Perez-GilabertF Garcia-Carmona
Mar 10, 1992·Biochemistry·K Ryu, J S Dordick
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R AffleckD S Clark
Jul 1, 1992·Biotechnology Progress·J S Dordick
Aug 14, 1992·Biochemical and Biophysical Research Communications·G Palazzo, P L Luisi
Sep 1, 1992·European Journal of Biochemistry·G Garza-RamosR W Gracy
May 24, 1991·Biochimica Et Biophysica Acta·A B HanleyA R Mackie
Jul 1, 1991·European Journal of Biochemistry·R Bru, P Walde
Dec 18, 1991·European Journal of Biochemistry·R Jaenicke
Aug 15, 1991·Biochemical and Biophysical Research Communications·Q Mao, P Walde
Jun 15, 1990·The Biochemical Journal·R BruF García-Carmona
Oct 24, 1990·Biochimica Et Biophysica Acta·M BonaM Sedlák
Mar 10, 1990·European Journal of Biochemistry·Q Q Peng, P L Luisi
Jan 12, 1990·European Journal of Biochemistry·R M VerhaertC Veeger
Jan 12, 1990·European Journal of Biochemistry·R M VerhaertC Veeger
Mar 17, 1986·European Journal of Biochemistry·K MartinekI V Berezin
Apr 1, 1989·Trends in Biochemical Sciences·A M Klibanov
Jun 6, 1989·Biochimica Et Biophysica Acta·K MartinekA V Levashov
Jun 30, 1989·Biochemical and Biophysical Research Communications·N PfammatterP L Luisi
Apr 15, 1989·The Biochemical Journal·R BruF Garcia-Carmona
Feb 24, 1988·Biochimica Et Biophysica Acta·P L LuisiB H Robinson
Jun 1, 1987·Photochemistry and Photobiology·K VosA J Visser

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Citations

Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·X G GaoA Rodríguez-Romero
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Gerhard Leubner-Metzger
Jul 22, 2010·International Journal of Biological Macromolecules·Yun-Yang WanJohn F Kennedy
Jan 14, 2009·Biophysical Chemistry·Rashid O AnarbaevOlga I Lavrik
May 27, 2008·Colloids and Surfaces. B, Biointerfaces·Yun ZhangPei-Ji Gao
Mar 29, 2005·Journal of Photochemistry and Photobiology. B, Biology·Rupa SarkarSamir Kumar Pal
Jun 30, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe CavallaroFilippo Parisi
Aug 22, 2001·FEBS Letters·R H van den HeuvelW J van Berkel
Nov 7, 2000·Biotechnology and Bioengineering·M García-GaribayE Barzana
Jan 5, 2017·Structure·Andrea G QuezadaMiguel Costas
Feb 22, 2002·Biotechnology and Bioengineering·N I Gerhardt, S R Dungan
Sep 18, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Wen-Bin OuHai-Meng Zhou
Jan 1, 2017·Organic Geochemistry·Mary Beth WilhelmAmy Williams
Apr 6, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Filip MeersmanKarel Heremans
Jan 28, 1999·Biochemical and Biophysical Research Communications·N L KlyachkoC Balny
Jul 14, 2001·Biochimica Et Biophysica Acta·G ZhuY Tang
Aug 26, 1999·International Journal of Pharmaceutics·W Wang
Sep 27, 2002·Current Opinion in Biotechnology·Frutos C Marhuenda-Egea, María José Bonete
Jun 16, 2012·Chemical Reviews·N Mariano CorreaNancy E Levinger
Apr 2, 2008·Journal of Colloid and Interface Science·Jun-Bao FanYi Liang
Feb 28, 2008·The Journal of Physical Chemistry. B·Norma Díaz-Vergara, Angel Piñeiro

❮ Previous
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