PMID: 15244065Jul 13, 2004Paper

Enzymes involved in carbohydrate metabolism and their role on exopolysaccharide production in Streptococcus thermophilus

Journal of Applied Microbiology
A EscalanteA Farrés

Abstract

The role of the enzymes uridine-5'-diphospho-(UDP) glucose pyrophosphorylase and UDP galactose 4-epimerase in exopolysaccharide production of Gal- ropy and non-ropy strains of Streptococcus thermophilus in a batch culture was investigated. Growth of the ropy and non-ropy strains was accompanied by total release of the galactose moiety from lactose hydrolysis in modified Bellinker broth with lactose as the only carbon source. This was associated with a greater exopolysaccharide production by the ropy strain. The polymer produced by both strains in cultures with lactose or glucose as carbon sources contained glucose, galactose and rhamnose, indicating that glucose was used as a carbon source for bacterial growth and for exopolysaccharide formation. UDP-glucose pyrophosphorylase activity was associated with polysaccharide production during the first 12 h in a 20 h culture in the ropy strain, but not in the non-ropy strain. UDP-galactose 4-epimerase was not associated with exopolysaccharide synthesis in any strain. The evidence presented suggests that the glucose moiety from lactose hydrolysis is the source of sugar for heteropolysaccharide synthesis, due to a high UDP-glucose pyrophosphorylase activity.

Citations

Jan 22, 2005·Applied Microbiology and Biotechnology·M I TorinoG Font de Valdez
Oct 20, 2009·Applied Microbiology and Biotechnology·Hui LiPing-Kai Ouyang
Mar 17, 2001·International Journal of Food Microbiology·A N HassanS I Shalabi
May 12, 2001·Journal of Applied Microbiology·N GorretJ M Engasser
Mar 31, 2017·Archives of Microbiology·Yanhua CuiTong Hu
Apr 19, 2007·Journal of Basic Microbiology·Anita Suresh KumarBhavanath Jha
Aug 24, 2017·FEMS Microbiology Reviews·Ahmad A ZeidanAna Rute Neves
May 11, 1999·FEMS Microbiology Reviews·L De Vuyst, B Degeest

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