PMID: 943182Feb 20, 1976Paper

Enzymic and immunochemical properties of lysozyme. XIII. Accurate delineation of the reactive site around the disulfide 6-127 by immunochemical study of beta-propiolactone lysozyme derivative and of synthetic disulfide peptides

Biochimica Et Biophysica Acta
M Z AtassiA F Habeeb

Abstract

In previous reports from this laboratory it was shown that an antigenic reactive site resides around the sequences 6-13 and 126-128 linked by the disulfide 6-127. The present work provides a strong support for the location of the reactive site by an independent approach. It also determines accurately the boundaries of the reactive site. 1. The two methionine residues in lysozyme were carboxyethylated by reaction with beta-propiolactone. The electrophoretically homogeneous derivative had no other modified amino acids and showed no conformational changes, relative to native lysozyme, as determined by ORD and CD measurements. However, it exhibited a slight increase in disulfide reducibility relative to native lysozyme and its lytic activity was about half that of native lysozyme, probably as a result of the slight conformational change. On the other hand, the antigenic reactivity of the derivative was equal to that of native lysozyme with several goat and rabbit antisera to lysozyem. It was therefore concluded that methionines 12 and 105 were not parts of antigenic reactive sites in native lysozyme. 2. Eleven peptides, corresponding to various sequences on the two sides of the disulfide 6-127 (i.e. two groups of peptides) were syn...Continue Reading

References

Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·P G Katsoyannis, A Tometsko
Mar 29, 1967·Journal of the American Chemical Society·F J Hartdegen, J A Rupley
Sep 1, 1956·Proceedings of the National Academy of Sciences of the United States of America·W Moffitt, J T Yang

Related Concepts

Metazoa
Antigen-Antibody Reactions
Circular Dichroism, Vibrational
Disulfides
Goat
Models, Structural
Leftose
Optical Rotatory Dispersion
Peptide Fragments
Polypeptides

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