Enzymic cross-linkage of monomeric extensin precursors in vitro.

Plant Physiology
D S EverdeenD T Lamport

Abstract

Rapidly growing tomato (Lycopersicon esculentum) cell suspension cultures contain transiently high levels of cell surface, salt-elutable, monomeric precursors to the covalently cross-linked extensin network of the primary cell wall. Thus, we purified a highly soluble monomeric extensin substrate from rapidly growing cells, and devised a soluble in vitro cross-linking assay based on Superose-6 fast protein liquid chromatography separation, which resolved extensin monomers from the newly formed oligomers within 25 minutes. Salt elution of slowly growing (early stationary phase) cells yielded little or no extensin monomers but did give a highly active enzymic preparation that specifically cross-linked extensin monomers in the presence of hydrogen peroxide, judging from: (a) a decrease in the extensin monomer peak on fast protein liquid chromatography gel filtration, (b) appearance of oligomeric peaks, and (c) direct electron microscopical observation of the cross-linked oligomers. The cross-linking reaction had a broad pH optimum between 5.5 and 6.5. An approach to substrate saturation of the enzyme required extensin monomer concentrations of 20 to 40 milligrams per milliliter. Preincubation with catalase completely inhibited the ...Continue Reading

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Citations

Sep 26, 1997·Molecular Plant-microbe Interactions : MPMI·J M ChittoorF F White
Feb 1, 1990·Plant Physiology·X B LiD T Lamport
Nov 19, 2015·The Plant Journal : for Cell and Molecular Biology·Cora A MacAlisterZachary B Lippman
Mar 4, 2014·The Plant Journal : for Cell and Molecular Biology·Mengmeng ZhuSixue Chen
Jan 27, 1989·Cell·J E Varner, L S Lin
Jan 18, 2006·The New Phytologist·Derek T A LamportAllan M Showalter
Jul 29, 2003·The Journal of Biological Chemistry·Nicholas J PricePhil A Jackson
May 31, 2001·Plant & Cell Physiology·S HiragaH Matsui
Nov 17, 2015·Biochemistry Insights·Yuning ChenMarcia J Kieliszewski
Oct 7, 2004·The Journal of Biological Chemistry·Michael A HeldMarcia J Kieliszewski
Dec 21, 2018·The Plant Journal : for Cell and Molecular Biology·Hidetaka KayaKazuyuki Kuchitsu
Feb 16, 2019·Journal of Neurosciences in Rural Practice·Martin MájovskýPavel Kučera
Sep 13, 2018·International Journal of Molecular Sciences·Derek T A LamportMarcia J Kieliszewski
May 12, 2020·The Plant Journal : for Cell and Molecular Biology·Steven BeuderCora A MacAlister
Mar 19, 2011·Plant Physiology·Derek T A LamportMaura C Cannon
Jun 1, 2021·Frontiers in Plant Science·John W Mishler-ElmoreMichael A Held

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