PMID: 8952457Nov 1, 1996Paper

Enzymic determinations with rotating bioreactors and continuous-flow-stopped-flow processing. Determination of choline esters in pharmaceuticals

The Analyst
B López RuizH A Mottola

Abstract

The neuromuscular blocking agent, succinylcholine, and the neurotransmitter, acetylcholine, have been determined in pharmaceutical preparations utilizing a sensing unit comprised of a rotating bioreactor and an amperometric detector. The bioreactor consisted of a rotating disk made of Teflon (1 cm id) with cholinesterase (EC 3.1.1.8) and choline oxidase (EC 1.1.3.17) co-immobilized on the top surface of the disk. Amperometric detection of H2O2 and initial rate measurements were employed for quantification.

References

Jun 1, 1975·Analytica Chimica Acta·K Gibson, G G Guilbault
Nov 30, 1990·Journal of Chromatography·Y IkarashiY Maruyama
Sep 16, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·L CampanellaM Tomassetti
Apr 1, 1969·Biochemical Pharmacology·I Hanin, D J Jenden
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Apr 1, 1984·Analytical Biochemistry·M L Gilberstadt, J A Russell
Apr 1, 1981·Journal of Neurochemistry·D R HaubrichM Zweig

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