PMID: 4290404Jun 1, 1966Paper

Enzymic hydrolysis of barley and other beta-glucans by a beta-(1--4)-glucan hydrolase

The Biochemical Journal
A E Clarke, B A Stone

Abstract

1. A barley glucan with 68% of beta-(1-->4)-linkages and 32% of beta-(1-->3)-linkages was exhaustively hydrolysed with an Aspergillus niger beta-(1-->4)-glucan 4-glucanohydrolase (EC 3.2.1.4) (Clarke & Stone, 1965b). The hydrolysis products were separated and estimated. 2. The lower-molecular-weight products were identified as: glucose, 1.4%; cellobiose, 11.9%; 3(2)-O-beta-glucosylcellobiose, 45.0%; a tetrasaccharide(s), which was a substituted cellobiose, 16.4%. A series of unidentified higher-molecular-weight products (26.5%) were also found. 3. The identity of the products suggests that the A. niger beta-(1-->4)-glucan hydrolase hydrolyses beta-glucosidic linkages joining 4-O-substituted glucose residues. 4. When an enzyme fraction containing the beta-(1-->4)-glucan hydrolase and an exo-beta-(1-->3)-glucan hydrolase was used, the same products were found, but the higher-molecular-weight products were observed to have only a transient existence in the hydrolysate and were virtually absent after prolonged incubation. It is suggested that these oligosaccharides are resistant to attack by beta-(1-->4)-glucan hydrolase but are partially hydrolysed by the exo-beta-(1-->3)-glucan hydrolase and therefore possess one or more (1-->3)-...Continue Reading

Citations

Jan 1, 1992·Nutrition Research Reviews·J M McNab, R R Smithard
Jan 1, 1989·Critical Reviews in Biotechnology·O P Ward, M Moo-Young
Jan 1, 1982·European Journal of Biochemistry·J R Woodward, G B Fincher
Apr 1, 1975·FEBS Letters·M A Anderson, B A Stone
Jun 16, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nirupama GangopadhyayNigel P Brunton
Feb 21, 2021·International Journal of Biological Macromolecules·Jéssica WoukAneli M Barbosa-Dekker
Jul 1, 1976·Carbohydrate Research·D J MannersJ Y Brodie
Jun 20, 1973·Biochimica Et Biophysica Acta·M M Smith, B A Stone
Mar 11, 1969·Biochimica Et Biophysica Acta·Z Nevo, N Sharon
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J Marshall, R J Grand

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