PMID: 832394Feb 1, 1977

Enzymic measurement of cholesterol in serum with the CentrifiChem centrifugal analyzer

Clinical Chemistry
M A Pesce, S H Bodourian


Cholesterol is measured by mixing 5 mul of sample with 350 mul of a reagent consisting of phenol, 4-aminoantipyrine, and the enzymes cholesterol oxidase, cholesterol esterase, and peroxidase. After 12 min, the resulting quinoneimine is measured at 520 nm. Readings and cholesterol concentrations are linearly related up to 4.0 g/liter. Lipemic sera and samples containing uric acid (up to 200 mg/liter), hemoglobin (up to 1 g/liter), and certain drugs (clofibrate, phenobarbital, nicotinic acid, Ketochol, Ovral-28), gave no interference. Abnormally high concentrations of bilirubin and ascorbic acid in serum lowered the cholesterol values. This enzymic assay, compared with the method of Abell and with a rate method that uses the Hantzsch reaction, gave correlation coefficients of 0.987 and 989, respectively.

Related Concepts

Bilirubin, (4E,15E)-Isomer
Lysosomal Acid Cholesterol Esterase
Hydroxysteroid Dehydrogenases

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