Enzymic thin film coatings for bioactive materials

Biotechnology Progress
Xiaodong TongPing Wang

Abstract

Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 microg/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.

Citations

Oct 4, 2011·Biotechnology Advances·Shakeel Ahmed Ansari, Qayyum Husain
Nov 3, 2010·Colloids and Surfaces. B, Biointerfaces·Menuk B JayawardenaStaffan J Kjelleberg
Mar 4, 2015·Journal of Biomaterials Science. Polymer Edition·Yibing WangPing Wang

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