PMID: 41535May 30, 1979

Enzymologic characterization of adenosine nucleosidase of medicinal plants (Medicago sativa)

Bollettino della Società italiana di biologia sperimentale
M CamiciP L Ipata

Abstract

Adenosine nucleosidase (adenosine ribohydrolase, E C 3.2.2.7) was purified from alfalfa leaf juice. The final preparation shows a single band on polyacrylamide gel electrophoresis; the enzyme activity is stable for 12 hrs between pH 5.5 and pH 8.5, but is completely lost on heating at 55 degrees C for 10 min. Optimal pH for the hydrolysis of adenosine is between pH 5 and pH 6. Among nine purine nucleosides tested, only adenosine, 2'-deoxyadenosine and purine riboside were hydrolyzed by the enzyme preparation. A Km value of 7 x 10(-6) M was found with adenosine as substrate at pH 7.4. Of the two reaction products, adenine exerted a weak inhibitory effect, while D-ribose was without effect on the initial rate of adenosine hydrolysis. The data reported are compared with those obtained on the enzymes from other plant sources.

Related Concepts

Exertion
Nebularine
Adenosine
Enzymes, antithrombotic
SDS-PAGE
Medicago sativa leaf extract
Enzyme agent
Enzyme Activity
Enzymes for Treatment of Wounds and Ulcers
Blood Enzyme Activity (Lab Test)

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