Enzymolysis of walnut (Juglans regia L.) meal protein: Ultrasonication-assisted alkaline pretreatment impact on kinetics and thermodynamics

Journal of Food Biochemistry
Moses Kwaku GollyMuhammad Farooq

Abstract

To improve the utility of walnut meal protein, influences of ultrasonication on the kinetic and thermodynamic constraints of enzyme (trypsin) hydrolysis were assessed using a multi-frequency counter current S-type ultrasound machine. Results revealed that both the ultrasonication treatment and control hydrolysis obeyed the first-order kinetics within the study constraints. After 90 min hydrolysis time, the uppermost percentage conversion rate (57.5%) of substrate to product and the percentage increase (25.42%) in hydrolyzed protein concentration were obtained at 323 K and 10% substrate concentration, respectively. The Michaelis constant (KM ) in ultrasonic enzymolysis declined by 58.66% over the control. The reaction rate coefficient (k) for the proteolysis improved by 84.75%, 52.43%, 48.25%, and 37.79% at 219, 303, 313, and 323 K, correspondingly. Generally, the bond energy (Ea ), enthalpy change (ΔH), entropy change (ΔS), and Gibbs free energy (ΔG) were reduced by the ultrasound pretreatment with 36.61%, 28.05%, 18.22%, and 5.24%, respectively. PRACTICAL APPLICATIONS: Walnut meal protein has a well-balanced amino acid profile and its economic utilization could be increased practicably as a food ingredient via production of hy...Continue Reading

References

Apr 17, 2007·Ultrasonics Sonochemistry·Rassoul Kadkhodaee, Malcolm J W Povey
Aug 17, 2010·Ultrasonics Sonochemistry·Haile MaWenxue Zhu
Jan 26, 2011·Ultrasonics Sonochemistry·Jayani ChandrapalaMuthupandian Ashokkumar
Jul 1, 2010·Nutrients·Emilio Ros
Feb 20, 2014·Journal of Food Science·Lidia López-BarriosSergio O Serna-Saldívar
Apr 8, 2015·Journal of the Science of Food and Agriculture·Jian JinYunliang Li
Sep 28, 2017·Ultrasonics Sonochemistry·Suyun LiYuqing Duan

❮ Previous
Next ❯

Citations

May 21, 2020·Journal of Food Science and Technology·Biswajit Biswas, Nandan Sit

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.