Eosinophil accumulation in postnatal lung is specific to the primary septation phase of development

Scientific Reports
Lucas F LoffredoS Berdnikovs

Abstract

Type 2 immune cells and eosinophils are transiently present in the lung tissue not only in pathology (allergic disease, parasite expulsion) but also during normal postnatal development. However, the lung developmental processes underlying airway recruitment of eosinophils after birth remain unexplored. We determined that in mice, mature eosinophils are transiently recruited to the lung during postnatal days 3-14, which specifically corresponds to the primary septation/alveolarization phase of lung development. Developmental eosinophils peaked during P10-14 and exhibited Siglec-Fmed/highCD11c-/low phenotypes, similar to allergic asthma models. By interrogating the lung transcriptome and proteome during peak eosinophil recruitment in postnatal development, we identified markers that functionally capture the establishment of the mesenchymal-epithelial interface (Nes, Smo, Wnt5a, Nog) and the deposition of the provisional extracellular matrix (ECM) (Tnc, Postn, Spon2, Thbs2) as a key lung morphogenetic event associating with eosinophils. Tenascin-C (TNC) was identified as one of the key ECM markers in the lung epithelial-mesenchymal interface both at the RNA and protein levels, consistently associating with eosinophils in developme...Continue Reading

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Citations

Aug 19, 2020·Nature Immunology·Juan M Inclan-RicoMark C Siracusa
Sep 7, 2021·Frontiers in Immunology·Josephine Schlosser-BrandenburgSusanne Hartmann

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Datasets Mentioned

BETA
GSE74243
GSE20954

Methods Mentioned

BETA
environmental stress
flow cytometry
bronchoalveolar lavage
RNA-Seq
PCR
PCA
bronchoalveolar
lavage
Flow

Software Mentioned

Treestar
R Bioconductor
R
GraphPad Prism
gplots
BioRender
ClueGO
cellSens Imaging
Bioconductor
Cytoscape

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