Eosinophil function in adipose tissue is regulated by Krüppel-like factor 3 (KLF3).

Nature Communications
Alexander J KnightsKate G R Quinlan

Abstract

The conversion of white adipocytes to thermogenic beige adipocytes represents a potential mechanism to treat obesity and related metabolic disorders. However, the mechanisms involved in converting white to beige adipose tissue remain incompletely understood. Here we show profound beiging in a genetic mouse model lacking the transcriptional repressor Krüppel-like factor 3 (KLF3). Bone marrow transplants from these animals confer the beige phenotype on wild type recipients. Analysis of the cellular and molecular changes reveal an accumulation of eosinophils in adipose tissue. We examine the transcriptomic profile of adipose-resident eosinophils and posit that KLF3 regulates adipose tissue function via transcriptional control of secreted molecules linked to beiging. Furthermore, we provide evidence that eosinophils may directly act on adipocytes to drive beiging and highlight the critical role of these little-understood immune cells in thermogenesis.

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Citations

Nov 10, 2020·Immunology and Cell Biology·Elizabeth A Jacobsen, Eleanna De Filippis
Dec 8, 2020·Frontiers in Cardiovascular Medicine·Ibrahim AlZaimAhmed F El-Yazbi
Feb 19, 2021·American Journal of Human Genetics·Victoria L WyckelsmaHåkan Westerblad
Apr 2, 2021·Circulation Research·Mascha KoenenJames R Sowers
Mar 2, 2021·Annual Review of Immunology·Elizabeth A JacobsenMarc E Rothenberg
Apr 26, 2021·The Journal of Physiology·William V TrimDylan Thompson
Aug 1, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer M FeltonMarc E Rothenberg
Aug 19, 2021·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·Christoph GranderHerbert Tilg
Sep 3, 2021·Cell Metabolism·Emily L GoldbergVishwa Deep Dixit
Oct 7, 2021·Annual Review of Cell and Developmental Biology·Conan J O O'BrienAna I Domingos
Nov 7, 2021·Nature Reviews. Immunology·W V Trim, L Lynch

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Methods Mentioned

BETA
transgenic
thermal stress
genotyping
biopsies
flow cytometry
FACS
ELISA
ChIP-Seq
ChIP
immunoprecipitations

Software Mentioned

Immgen
Illustrator
Integrative Genomics Viewer
Biosystems QuantStudio Real - Time PCR
GraphPad Prism
BioRender
FlowJo
Applied
Bio
Partek Genomics Suite

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