Eosinophils Modulate CD4(+) T Cell Responses via High Mobility Group Box-1 in the Pathogenesis of Asthma

Allergy, Asthma & Immunology Research
Eun-Jin ShimHeung-Woo Park

Abstract

Eosinophils have been reported to modulate T cell responses. Previously, we reported that high-mobility group box 1 protein (HMGB1) played a key role in the pathogenesis of asthma. This study was conducted to test our hypothesis that eosinophils could modulate T cell responses via HMGB1 in the pathogenesis of asthma characterized by eosinophilic airway inflammation. We performed in vitro experiments using eosinophils, dendritic cells (DCs), and CD4(+) T cells obtained from a murine model of asthma. The supernatant of the eosinophil culture was found to significantly increase the levels of interleukin (IL)-4 and IL-5 in the supernatant of CD4(+) T cells co-cultured with DCs. HMGB1 levels increased in the supernatant of the eosinophil culture stimulated with IL-5. Anti-HMGB1 antibodies significantly attenuated increases of IL-4 and IL-5 levels in the supernatant of CD4(+) T cells co-cultured with DCs that were induced by the supernatant of the eosinophil culture. In addition, anti-HMGB1 antibodies significantly attenuated the expressions of activation markers (CD44 and CD69) on CD4(+) T cells. Our data suggest that eosinophils modulate CD4(+) T cell responses via HMGB1 in the pathogenesis of asthma.

References

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Citations

Mar 31, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Chris GraingeFanny W S Ko
May 20, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ji Hyun SongMinhyung Lee
Jul 12, 2020·Journal of Basic and Clinical Physiology and Pharmacology·Farzaneh KianianBehjat Seifi
Jun 21, 2017·Clinical and Molecular Allergy : CMA·Egidio ImbalzanoSebastiano Gangemi

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Methods Mentioned

BETA
density gradient centrifugation
ELISA
flow cytometry

Software Mentioned

CellQuest
Cell Quest

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