Eosinophils retain their granule major basic protein in a murine model of allergic pulmonary inflammation

American Journal of Respiratory Cell and Molecular Biology
D SteltsM Minnicozzi

Abstract

Accumulation of eosinophils in the lung with concomitant tissue damage are defining histopathologic features of human asthma. Through degranulation and the release of proinflammatory proteins such as major basic protein (MBP), eosinophils may perpetuate this inflammatory response. We investigated the extent of eosinophil degranulation in a murine model of allergic pulmonary inflammation. In this paradigm, the mice develop pulmonary eosinophilia, mucus hypersecretion, tissue damage, and airway edema and hyperreactivity. To evaluate the degree of eosinophil degranulation, we used a polyclonal antibody to murine MBP (mMBP) to perform dot blot analysis of bronchoalveolar lavage (BAL) cells and fluids, and immunohistochemical fluorescent analysis of lung tissue sections. After ovalbumin antigen challenge, we were unable to detect immunoreactive mMBP in the BAL fluids from either nonsensitized or sensitized mice. However, after lysis of the recoverable BAL cells, we were able to detect mMBP by immunoblot analysis, with the levels of immunoreactive mMBP directly related to the number of recoverable eosinophils. We also examined paraffin-embedded, lung tissue sections for patterns of mMBP deposition. Whereas lung sections from allergic...Continue Reading

References

Dec 1, 1992·Journal of Leukocyte Biology·R I Abu-GhazalehG J Gleich
Jan 1, 1992·Advances in Immunology·J P Arm, T H Lee
Jan 1, 1992·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·T M Litchfield, T H Lee
Mar 1, 1991·The American Review of Respiratory Disease·S T HolgateM K Church
Apr 1, 1991·The Journal of Clinical Investigation·R H GundelG J Gleich
Apr 1, 1986·The Journal of Allergy and Clinical Immunology·E Frigas, G J Gleich
Sep 1, 1987·The Journal of Allergy and Clinical Immunology·G J GleichL P Kravis
Jan 1, 1981·Journal of Immunological Methods·W V FilleyG J Gleich
Mar 1, 1995·Immunology Today·P J Barnes, F Y Liew
Sep 1, 1995·American Journal of Respiratory Cell and Molecular Biology·T T KungR W Chapman
Apr 1, 1995·American Journal of Respiratory Cell and Molecular Biology·T T KungR W Chapman
Jun 1, 1995·The Journal of Clinical Investigation·M KanekoH Kita
Jun 1, 1994·American Journal of Respiratory Cell and Molecular Biology·S H GavettM Wills-Karp
Sep 1, 1994·International Archives of Allergy and Immunology·T T KungA S Watnick
Feb 1, 1994·The Journal of Experimental Medicine·S DubucquoiM Capron
Apr 1, 1993·The American Review of Respiratory Disease·T OhnishiG J Gleich
Dec 7, 1995·European Journal of Pharmacology·E M HesselF P Nijkamp
Sep 1, 1996·American Journal of Respiratory Cell and Molecular Biology·C G GarlisiS P Umland
Mar 1, 1997·American Journal of Respiratory Cell and Molecular Biology·H ShiC Zhang
Mar 3, 1997·The Journal of Experimental Medicine·M KorsgrenC G Persson
Nov 5, 1997·American Journal of Respiratory Cell and Molecular Biology·L S FederT T Kung

❮ Previous
Next ❯

Citations

Sep 20, 1976·Biochimica Et Biophysica Acta·D R Coventry, M J Dilworth
Jul 13, 2002·Pharmacology & Therapeutics·Y Riffo-Vasquez, D Spina
Jul 30, 2003·Free Radical Biology & Medicine·Athena A AndreadisSerpil C Erzurum
Aug 31, 2001·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·K P Leong, D P Huston
Aug 15, 2000·The International Journal of Biochemistry & Cell Biology·Y Riffo-VasquezD Spina
Dec 22, 1999·Allergy·C G Persson, J S Erjefält
Dec 12, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P AssanasenR M Naclerio
Feb 2, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Revital ShamriPeter F Weller
May 17, 2000·Current Opinion in Pulmonary Medicine·M F Lipscomb, J A Wilder
Jun 14, 2000·American Journal of Respiratory and Critical Care Medicine·J S Erjefält, C G Persson
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·A TomkinsonE W Gelfand
Oct 24, 2001·American Journal of Respiratory and Critical Care Medicine·A DuguetD H Eidelman
Apr 17, 2002·American Journal of Respiratory and Critical Care Medicine·Azzeddine DakhamaErwin W Gelfand
Feb 6, 2003·American Journal of Respiratory and Critical Care Medicine·Gloria C KooIrwin I Singer
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·M Malm-ErjefältJ S Erjefält
Nov 22, 2002·American Journal of Respiratory Cell and Molecular Biology·Stephania A CormierJames J Lee
Sep 3, 2002·American Journal of Respiratory Cell and Molecular Biology·Rakesh K Kumar, Paul S Foster
Jun 8, 2012·Journal of Leukocyte Biology·Valdirene S MunizJosiane S Neves
Sep 1, 2012·The Journal of Allergy and Clinical Immunology·James J LeeNancy A Lee
May 10, 2011·Journal of Ethnopharmacology·Dae Yong Kim, Woong Mo Yang
Sep 20, 2000·Redox Report : Communications in Free Radical Research·S N MitraS L Hazen
Sep 12, 2013·International Immunopharmacology· SubhashiniRashmi Singh
Dec 4, 2013·Blood·Caroline M PercopoHelene F Rosenberg
Dec 7, 2016·American Journal of Respiratory and Critical Care Medicine·Elizabeth A JacobsenNancy A Lee
Aug 27, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J J Lee, N A Lee
Jun 19, 2017·The Journal of Allergy and Clinical Immunology·Njira LugogoJulie G Ledford
Jun 26, 2017·Journal of Immunological Methods·Kiho SonParameswaran Nair
May 3, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y Riffo-VasquezC P Page
Aug 30, 2013·Journal of Leukocyte Biology·John Dongil KimPaige Lacy
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joel N KlineVipul V Jain
Jul 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Noel Verjan GarciaMasayuki Miyasaka
Jan 17, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Paul JusticeM R Van Scott
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K L DenzlerN A Lee
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takao KobayashiHirohito Kita
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·M R van ScottS Sur
Jun 7, 2019·International Journal of Molecular Sciences·Ryoji ItoSatoshi Nunomura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Related Papers

American Journal of Respiratory Cell and Molecular Biology
M Malm-ErjefältJ S Erjefält
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
K Roberts, Z Jaffar
International Archives of Allergy and Applied Immunology
W ChenB W Manktelow
© 2022 Meta ULC. All rights reserved