EP24.15 interacts with the angiotensin II type I receptor and bradykinin B2 receptor

Cell Biochemistry and Function
Bangalore R ShivakumarRaymond C Harris

Abstract

The carboxyl-terminal cytoplasmic domain of the angiotensin II type 1 receptor (AT1) is known to interact with several classes of intracellular proteins that may modulate receptor function. Employing yeast two-hybrid screening of a human embryonic kidney cDNA library with the carboxyl-terminal cytoplasmic domain of the AT1 receptor as a bait, we have isolated EP24.15 (EC 3.4.24.15, thimet oligopeptidase) as a potentially interacting protein. EP24.15 is widely distributed and is known to degrade bioactive peptides such as angiotensin I and II and bradykinin. In addition, EP24.15 was previously identified as a putative soluble angiotensin II binding protein. Two-hybrid screening also determined that EP24.15 can interact with the B2 bradykinin receptor. Transient expression of EP24.15 in a porcine kidney epithelial cell line stably expressing full length AT1 and full length B2 followed by affinity chromatography and co-immunoprecipitation confirmed EP24.15 association with both AT1 and B2 receptors. EP24.15 was also co-immunoprecipitated with AT1 and B2 in rat kidney brush border membranes (BBM) and basolateral membranes (BLM). Both AT1 and B2 undergo ligand-induced endocytosis. Analysis of endosomal fractions following immunoprec...Continue Reading

References

Oct 1, 1992·Kidney International·T G Hammond
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1982·The American Journal of Physiology·K Amsler, J S Cook
Dec 16, 1980·Biochemical and Biophysical Research Communications·E J GoetzlB U Wintroub
Jun 1, 1994·The American Journal of Physiology·T G Hammond, P J Verroust
Apr 1, 1995·The American Journal of Physiology·K D Burns, R C Harris
Jan 1, 1995·Experimental Cell Research·J M ChenA J Barrett
Jan 1, 1997·The American Journal of Physiology·H Van RijenH J Jongsma
Sep 20, 1997·The Journal of Biological Chemistry·T SanoT Inagami
Apr 29, 1998·The Journal of Biological Chemistry·R C VenemaM B Marrero
Sep 3, 1998·The Journal of Biological Chemistry·H JuR C Venema
Jan 7, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M C ChappellC M Ferrario
Jan 8, 1999·Regulatory Peptides·C M Ferrario, S N Iyer
Jul 16, 1999·Clinical and Experimental Pharmacology & Physiology·T InagamiT Yamakawa
Sep 18, 2001·The Journal of Comparative Neurology·J D Fontenele-NetoE S Ferro
Oct 20, 2001·Cell Research·D F GuoT Inagami
Oct 30, 2001·The Journal of Biological Chemistry·Jennifer L SeachristStephen S G Ferguson
Oct 10, 2002·Endocrine Reviews·Corie N ShrimptonRebecca A Lew
Feb 15, 2003·American Journal of Physiology. Heart and Circulatory Physiology·M Ursula NormanMichael J Hickey

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Citations

Jan 4, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Robert C Speth, Vardan T Karamyan
May 17, 2012·Pflügers Archiv : European journal of physiology·Hayo Castrop
Mar 6, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Jun IwanamiMasatsugu Horiuchi
Jan 23, 2010·Journal of the American Society of Nephrology : JASN·Mona OppermannHayo Castrop
May 12, 2007·Regulatory Peptides·Vardan T Karamyan, Robert C Speth
Aug 25, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Masaki MogiMasatsugu Horiuchi
Apr 24, 2008·The Journal of Pharmacology and Experimental Therapeutics·Caroline SandénL M Fredrik Leeb-Lundberg
Apr 16, 2010·Physiological Reviews·Hayo CastropCharlotte Wagner
May 12, 2009·Current Opinion in Nephrology and Hypertension·Masaki MogiMasatsugu Horiuchi
Feb 24, 2012·American Journal of Physiology. Renal Physiology·Elisabeth DoblingerHayo Castrop

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