Eph signaling controls mitotic spindle orientation and cell proliferation in neuroepithelial cells

The Journal of Cell Biology
Maribel Franco, Ana Carmena

Abstract

Mitotic spindle orientation must be tightly regulated during development and adult tissue homeostasis. It determines cell-fate specification and tissue architecture during asymmetric and symmetric cell division, respectively. Here, we uncover a novel role for Ephrin-Eph intercellular signaling in controlling mitotic spindle alignment in Drosophila optic lobe neuroepithelial cells through aPKC activity-dependent myosin II regulation. We show that conserved core components of the mitotic spindle orientation machinery, including Discs Large1, Mud/NuMA, and Canoe/Afadin, mislocalize in dividing Eph mutant neuroepithelial cells and produce spindle alignment defects in these cells when they are down-regulated. In addition, the loss of Eph leads to a Rho signaling-dependent activation of the PI3K-Akt1 pathway, enhancing cell proliferation within this neuroepithelium. Hence, Eph signaling is a novel extrinsic mechanism that regulates both spindle orientation and cell proliferation in the Drosophila optic lobe neuroepithelium. Similar mechanisms could operate in other Drosophila and vertebrate epithelia.

References

Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·T L JonesI O Daar
Jun 5, 1999·Molecular and Cellular Neurosciences·A L ScullyJ B Thomas
Apr 18, 2000·The Journal of Cell Biology·S WahlB K Mueller
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·M P Scheid, J R Woodgett
Oct 31, 2003·Cancer Cell·Ji LuoLewis C Cantley
Apr 20, 2004·Cellular Signalling·Nicole K Noren, Elena B Pasquale
Mar 29, 2005·Nature Cell Biology·Zhong LiDianqing Wu
Aug 16, 2005·Cell Metabolism·Noboru FurukawaYoung-Bum Kim
Jun 3, 2006·Developmental Cell·Sarah K BowmanJuergen A Knoblich
Sep 26, 2007·The Journal of Cell Biology·Morgan B GouldingBruce Bowerman
Feb 13, 2008·The Journal of Cell Biology·Graham J ButtrickJames G Wakefield
Jun 11, 2008·The Journal of Cell Biology·Runxiang QiuQiang Lu
Jul 8, 2008·Nature Cell Biology·Hyun-Shik LeeIra O Daar
Aug 30, 2008·Cell Cycle·Graham J Buttrick, James G Wakefield
Mar 12, 2009·Cell Adhesion & Migration·Hyun-Shik Lee, Ira O Daar
Dec 28, 2010·Current Biology : CB·Mihaela ŽigmanCecilia B Moens
Feb 9, 2011·The Journal of Biological Chemistry·Joanne DurganAlan Hall
Mar 16, 2011·Journal of Cell Science·Jillian C Pease, Jennifer S Tirnauer
Jul 19, 2011·Developmental Cell·Xavier Morin, Yohanns Bellaïche
Jul 26, 2011·Trends in Cell Biology·Luke Martin McCaffrey, Ian G Macara
Aug 3, 2011·Seminars in Cell & Developmental Biology·Adam D Werts, Bob Goldstein
Oct 18, 2011·Current Opinion in Cell Biology·Irinka Castanon, Marcos González-Gaitán
Oct 26, 2011·The Journal of Cell Biology·Brett WeeChris Q Doe

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Citations

Sep 3, 2019·Cell Cycle·Maribel Franco, Ana Carmena
Oct 8, 2019·Molecular Biology and Evolution·Aida ArcasM Ángela Nieto
Dec 23, 2020·Current Biology : CB·Scott E Williams, Kendall J Lough

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ImageJ
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