EphB2 regulates axonal growth at the midline in the developing auditory brainstem

Developmental Biology
Karina S CramerShazia A Siddiqui

Abstract

Eph receptors play important roles in axon guidance at the midline. In the auditory system, growth of axons across the midline is an important determinant of auditory function. The avian cochlear nucleus, n. magnocellularis (NM), makes bilateral projections to its target, n. laminaris (NL). We examined the time course of NM axon growth toward the midline, the expression of Eph proteins at the midline during this growth, and the effects of Eph receptor misexpression on axonal growth across the midline. We found that NM axons reach the midline at E4. At this age, EphB receptors are expressed at the ventral floor plate. Expression extends dorsally to the ventricular zone beginning at E5. NM axons thus grow across the midline at a time when EphB receptor expression levels are low. Overexpression of EphB2 at E2 resulted in misrouted axons that deflected away from transfected midline cells. This effect was observed when midline cells were transfected but not when NM cells alone were transfected, suggesting that EphB2 acts non-cell autonomously and through reverse signaling. These data suggest an inhibitory role for midline Eph receptors, in which low levels permit axon growth and subsequently high levels prohibit growth after axons h...Continue Reading

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Citations

Nov 8, 2008·Journal of Visualized Experiments : JoVE·Matthew J Korn, Karina S Cramer
Jul 28, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Candace Y HsiehKarina S Cramer
Aug 23, 2012·Neural Development·Michelle R Allen-Sharpley, Karina S Cramer
Nov 3, 2011·Seminars in Cell & Developmental Biology·Nan-Jie Xu, Mark Henkemeyer
Jun 9, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·H KudoM Kaeriyama
Oct 31, 2007·Hearing Research·Ilona J MikoKarina S Cramer
Aug 21, 2007·The Journal of Comparative Neurology·David M HowellPeter H Mathers
Dec 15, 2012·The Journal of Comparative Neurology·Daniel T KashimaArmin H Seidl
Oct 25, 2011·Developmental Neurobiology·Paul A NakamuraKarina S Cramer
Oct 22, 2016·Neuroscience and Biobehavioral Reviews·Kathryn D BakerRick Richardson
Apr 20, 2016·F1000Research·Karina S Cramer, Ilona J Miko
Jun 20, 2007·Developmental Neurobiology·Kelly J Huffman, Karina S Cramer
Aug 21, 2007·The Journal of Comparative Neurology·Candace Y HsiehKarina S Cramer
Oct 24, 2007·The Journal of Comparative Neurology·Ilona J MikoKarina S Cramer
May 24, 2008·Developmental Neurobiology·Matthew J Korn, Karina S Cramer

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