In this study, we investigate the possible role of ephrin-Eph signaling in trigeminal motor axon projections. We find that EphA receptors are expressed at higher levels by rhombomere 2 (r2) trigeminal motor neurons than by r3 trigeminal motor neurons in the chick embryo. Mapping of rhombomere-specific axon projections shows that r2 and r3 trigeminal motor neurons project to different muscle targets, including the mandibular adductor and the intermandibularis muscles respectively. Ephrin-A5 is expressed in these muscles, especially in some regions of the intermandibularis muscle, and can cause growth cone collapse of both r2 and r3 motor axons in vitro. We demonstrate that in vivo overexpression of ephrin-A5 in the intermandibularis muscle, or overexpression of dominant-negative EphA receptors in trigeminal motor neurons leads to a reduction in branching of r3-derived motor axons specifically. Overexpression of full-length EphA receptors impairs the formation of r3 projections to the intermandibularis muscle. These findings indicate that ephrins and their Eph receptors play a role in trigeminal motor axon topographic mapping and in rhombomere 3-derived projections in particular.
Central representation and somatotopic organization of the jaw muscles within the facial and trigeminal nuclei of the pigeon (Columba livia)
In vitro guidance of retinal ganglion cell axons by RAGS, a 25 kDa tectal protein related to ligands for Eph receptor tyrosine kinases
E-box- and MEF-2-independent muscle-specific expression, positive autoregulation, and cross-activation of the chicken MyoD (CMD1) promoter reveal an indirect regulatory pathway.
Motor components of the trigeminal nerve and organization of the mandibular arch muscles in vertebrates. Phylogenetically conservative patterns and their ontogenetic basis
Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo.
A chick homologue of Serrate and its relationship with Notch and Delta homologues during central neurogenesis
Cranial motor axons respond differently to the floor plate and sensory ganglia in collagen gel co-cultures
The receptor tyrosine kinase, Cek8, is transiently expressed on subtypes of motoneurons in the spinal cord during development
Expression of the Tyro4/Mek4/Cek4 gene specifically marks a subset of embryonic motor neurons and their muscle targets
Differential expression of LIM homeobox genes among motor neuron subpopulations in the developing chick brain stem
Differentiation of avian craniofacial muscles: I. Patterns of early regulatory gene expression and myosin heavy chain synthesis
Topographic targeting and pathfinding errors of retinal axons following overexpression of ephrinA ligands on retinal ganglion cell axons
Expression of Eph receptor tyrosine kinases and their ligands in chick embryonic motor neurons and hindlimb muscles
Eph receptors and ephrin expression in cranial motor neurons and the branchial arches of the chick embryo
Expression of EphA4, ephrin-A2 and ephrin-A5 during axon outgrowth to the hindlimb indicates potential roles in pathfinding
Coordinate roles for LIM homeobox genes in directing the dorsoventral trajectory of motor axons in the vertebrate limb
The EphA4 receptor tyrosine kinase is necessary for the guidance of nasal retinal ganglion cell axons in vitro
Somatic motoneurone specification in the hindbrain: the influence of somite-derived signals, retinoic acid and Hoxa3
Topographic motor projections in the limb imposed by LIM homeodomain protein regulation of ephrin-A:EphA interactions
Cadherin-7 and cadherin-6B differentially regulate the growth, branching and guidance of cranial motor axons
Embryologic innervation of the rat laryngeal musculature--a model for investigation of recurrent laryngeal nerve reinnervation
Stromal cell-derived factor-1 and hepatocyte growth factor guide axon projections to the extraocular muscles
Development of the trigeminal motor neurons in parrots: implications for the role of nervous tissue in the evolution of jaw muscle morphology
Insulin-like signaling negatively regulates muscle arm extension through DAF-12 in Caenorhabditis elegans
Axon guidance in the developing ocular motor system and Duane retraction syndrome depends on Semaphorin signaling via alpha2-chimaerin
Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.
Advanced Imaging of Cellular Signaling
Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.