Ephrin-B2 elicits differential growth cone collapse and axon retraction in retinal ganglion cells from distinct retinal regions.

Developmental Neurobiology
Timothy J PetrosCarol Mason

Abstract

The circuit for binocular vision and stereopsis is established at the optic chiasm, where retinal ganglion cell (RGC) axons diverge into the ipsilateral and contralateral optic tracts. In the mouse retina, ventrotemporal (VT) RGCs express the guidance receptor EphB1, which interacts with the repulsive guidance cue ephrin-B2 on radial glia at the optic chiasm to direct VT RGC axons ipsilaterally. RGCs in the ventral retina also express EphB2, which interacts with ephrin-B2, whereas dorsal RGCs express low levels of EphB receptors. To investigate how growth cones of RGCs from different retinal regions respond upon initial contact with ephrin-B2, we utilized time-lapse imaging to characterize the effects of ephrin-B2 on growth cone collapse and axon retraction in real time. We demonstrate that bath application of ephrin-B2 induces rapid and sustained growth cone collapse and axon retraction in VT RGC axons, whereas contralaterally-projecting dorsotemporal RGCs display moderate growth cone collapse and little axon retraction. Dose response curves reveal that contralaterally-projecting ventronasal axons are less sensitive to ephrin-B2 treatment compared to VT axons. Additionally, we uncovered a specific role for Rho kinase signaling...Continue Reading

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Citations

Nov 13, 2012·Nature Neuroscience·Michael J SoskisMichael E Greenberg
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stéphane NédelecHynek Wichterle
Aug 30, 2014·Trends in Neurosciences·Sarah E Pease, Rosalind A Segal
Sep 24, 2013·Developmental Biology·Maëva LuxeyAlice Davy
Apr 23, 2013·Current Biology : CB·Jean-Michel CioniFabrice Ango
Oct 12, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Eloísa HerreraVerónica Murcia-Belmonte
Aug 5, 2017·Annual Review of Neuroscience·Tania A SeabrookAndrew D Huberman
Oct 21, 2019·Journal of Neurochemistry·Sergi Roig-PuiggrosAlain Chédotal
Oct 18, 2018·Nature Communications·Marianna MadeoPaola D Vermeer
May 13, 2020·Annual Review of Vision Science·Carol Mason, Nefeli Slavi

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