Jul 7, 2005

Ephrin-B2 ligand is a functional receptor for Hendra virus and Nipah virus

Proceedings of the National Academy of Sciences of the United States of America
Matthew I BonaparteChristopher C Broder

Abstract

Hendra virus (HeV) and Nipah virus (NiV) belong to the genus Henipavirus of the family Paramyxoviridae and are unique in that they exhibit a broad species tropism and cause fatal disease in both animals and humans. They infect cells through a pH-independent membrane fusion process mediated by their fusion and attachment glycoproteins. Previously, we demonstrated identical cell fusion tropisms for HeV and NiV and the protease-sensitive nature of their unknown cell receptor and identified a human cell line (HeLa-USU) that was nonpermissive for fusion and virus infection. Here, a microarray analysis was performed on the HeLa-USU cells, permissive HeLa-CCL2 cells, and two other permissive human cell lines. From this analysis, we identified a list of genes encoding known and predicted plasma membrane surface-expressed proteins that were highly expressed in all permissive cells and absent from the HeLa-USU cells and rank-ordered them based on their relative levels. Available expression vectors containing the first 10 genes were obtained and individually transfected into HeLa-USU cells. One clone, encoding human ephrin-B2 (EFNB2), was found capable of rendering HeLa-USU cells permissive for HeV- and NiV-mediated cell fusion as well as...Continue Reading

Mentioned in this Paper

Tumor Virus Infections
Nipah Virus
Microarray Analysis
Shuttle Vectors
Virus Diseases
expression vector
Glycoproteins
EFNB2 gene
Monocyte Chemoattractant Protein-1
Hendra Virus

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