EphrinB-EphB signaling regulates spinal pain processing via PKCγ

Neuroscience
X-L ZhouM Yan

Abstract

Spinal ephrinB-EphB signaling is involved in the modulation of pain processing. The aim of the present study was to investigate whether protein kinase C-γ (PKCγ) acts as a downstream effector in regulating spinal pain processing associated with ephrinB-EphB signaling in mice. The intrathecal injection of ephrinB2-Fc, an EphB receptor activator, caused thermal hyperalgesia and mechanical allodynia, as well as increased activation of spinal PKCγ. Knockdown of spinal PKCγ prevented the pain behaviors induced by ephrinB2-Fc. Furthermore, the intrathecal injection of EphB2-Fc, an EphB receptor blocker, suppressed formalin-induced inflammatory, chronic constriction injury (CCI)-induced neuropathic, and tibia bone cavity tumor cell implantation (TCI)-induced bone cancer pain behaviors, in addition to reducing the activation of spinal PKCγ. Finally, the intrathecal injection of MK801, an N-methyl-D-aspartate (NMDA) receptor blocker, prevented the pain behaviors and spinal PKCγ activation induced by ephrinB2-Fc. Overall, the results confirm the important role of PKCγ in the regulation of spinal pain processing associated with ephrinB-EphB signaling.

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Citations

Oct 12, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lei ZhangXiaohua Hou
May 10, 2020·Anesthesia and Analgesia·Juan P CataHolger K Eltzschig
Jan 11, 2019·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ravneet Kaur KhanguraAmteshwar Singh Jaggi
Feb 20, 2018·Experimental and Therapeutic Medicine·Jin-Shan YangGang Wu
Feb 29, 2020·Journal of Neural Transmission·Alain ArtolaRadhouane Dallel
Oct 19, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jia SunDa-Wei Ye
Jul 22, 2018·Molecular and Cellular Neurosciences·Nathan T Henderson, Matthew B Dalva

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