EphrinB/EphB signaling contributes to spinal nociceptive processing via calpain‑1 and caspase‑3.

Molecular Medicine Reports
Mei YangHongbin Yuan

Abstract

Previous studies have indicated that an important subfamily of receptor tyrosine kinases, ephrins and their receptors, are important in pain signaling, particularly in spinal nociceptive processing. In the present study, the role of the ephrin/Eph signaling pathway was confirmed, and it was shown that this signaling was also involved in spinal nociceptive processing through the actions of calpain‑1 and caspase‑3. First, the ephrinB ligands, ephrinB1‑Fc or ephrinB2‑Fc, were introduced into experimental mice via intrathecal injection, and it was found that this injection induced marked time‑ and dose‑dependent mechanical allodynia and thermal hyperalgesia, accompanied by increased levels of calpain‑1 and caspase‑3 in the spinal cord. MDL28170, an inhibitor of calpain‑1, reversed the behavioral effects and ameliorated the increases in calpain‑1 and caspase‑3. Second, it was found that the administration of EphB1 between L5 and L6 in mice inhibited the mechanical allodynia and thermal hyperalgesia induced by chronic constrictive injury. In addition, to demonstrate the cell phenotypes responsible for the increased levels of calpain‑1 and caspase‑3 in the spinal cord following injection with ephrinB2‑Fc, double immunofluorescent labe...Continue Reading

References

Oct 17, 1980·European Journal of Pharmacology·J L Hylden, G L Wilcox
Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
Jan 23, 1999·Cell Death and Differentiation·M I Pörn-AresS Orrenius
Sep 24, 1999·Journal of Neuroscience Research·S L Chan, M P Mattson
Mar 18, 2003·Nature Neuroscience·Anna Aurora BattagliaIsabella Gavazzi
Jun 21, 2005·Nature Neuroscience·Casper C HoogenraadMorgan Sheng
Mar 8, 2007·Annual Review of Neuroscience·Zu-Lin ChenSidney Strickland
Nov 26, 2008·Nature Neuroscience·Rüdiger Klein
Jul 15, 2009·Head & Neck Oncology·Joel B EpsteinDana Villines
Apr 22, 2010·Neuroreport·Kunihiro NakaiKoichi Ueda
Oct 16, 2010·Nature Medicine·Rohini Kuner
Nov 10, 2010·Molecular Pain·Jing ZhaoJohn N Wood
Mar 30, 2011·Molecular Pain·Ruth RuscheweyhJürgen Sandkühler
Jun 10, 2011·Communicative & Integrative Biology·Zhiye ZhuangDaniel J Liebl
Jul 20, 2012·Continuum : Lifelong Learning in Neurology·Nadine Attal
Mar 29, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mandana AminiDavid S Park
May 26, 2016·Molecular Medicine Reports·Xinhui YangQiusheng Zheng

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Methods Mentioned

BETA
electrophoresis

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GraphPad Prism
Image J

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