Epicardial cells derived from human embryonic stem cells augment cardiomyocyte-driven heart regeneration.

Nature Biotechnology
Johannes BargehrSanjay Sinha

Abstract

The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts in infarcted athymic rat hearts. Epicardial cells markedly enhanced the contractility, myofibril structure and calcium handling of human engineered heart tissues, while reducing passive stiffness compared with mesenchymal stromal cells. Transplanted epicardial cells formed persistent fibroblast grafts in infarcted hearts. Cotransplantation of hESC-derived epicardial cells and cardiomyocytes doubled graft cardiomyocyte proliferation rates in vivo, resulting in 2.6-fold greater cardiac graft size and simultaneously augmenting graft and host vascularization. Notably, cotransplantation improved systolic function compared with hearts receiving either cardiomyocytes alone, epicardial cells alone or vehicle. The ability of epicardial cells to enhance cardiac graft size and function makes them a promising adjuvant therapeutic for cardiac repair.

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Datasets Mentioned

BETA
GSE122714

Methods Mentioned

BETA
flow cytometry
PCR
Fluorescence
RNA-Seq

Software Mentioned

made4
FACSDiva
heatplot
ZEN
WebGestalt
QuckGO
HISAT2
GraphPad Prism
DESeq2
Image J

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