Epidemiological and quantitative study of GBV-C infection in french polytransfused children

Journal of Medical Virology
S CastelainGilles Duverlie

Abstract

From 1999 to 2002, 246 serum samples taken from polytransfused children were tested for the presence of GB virus C (GBV-C) RNA using a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. This assay was based on the TaqMan technology and allowed viral load determination in infected children with a dynamic range from 10(3) to 10(7) genome equivalent (gEq) copies/ml. The limit of detection was estimated to 619 gEq copies/ml with a > or = 95% probability of a positive result. Thirty five sera were found to be GBV-C RNA positive, corresponding to a prevalence of GBV-C of 14.2%. The mean viral load was high, i.e., 6 +/- 1.4 log (range 3.22-7.42) gEq copies/ml, but low viral loads were also detected. Sequencing of the 5'-untranslated region (UTR) identified a majority of genotype 2 strains (82%) distributed into two subtypes, 88.5% genotype 2a and 11.5% genotype 2b. In conclusion, GBV-C active infection is very frequent in exposed populations such as polytransfused children. GBV-C RNA quantitation using real-time assay may be useful for diagnosis and follow-up of the natural history of GBV-C infection.

References

Nov 1, 1996·Journal of Clinical Microbiology·V SchlueterA M Engel
Jul 1, 1997·European Journal of Pediatrics·J L ChungT Y Lin
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M G PessoaT L Wright
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T BergE Schreier
Aug 25, 1999·Journal of Medical Virology·H KomatsuM Inui
Aug 10, 2000·Journal of Medical Virology·T J Tucker, H E Smuts
Apr 18, 2001·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y H HuangT C Chang
Dec 26, 2001·Bioinformatics·S KumarM Nei
Feb 28, 2002·The Journal of Infectious Diseases·Sandrine CastelainUNKNOWN Agence Nationale de Recherche sur le SIDA AC11 Group
Mar 12, 2003·Journal of Medical Virology·A Scott MuerhoffSuresh M Desai
Mar 12, 2003·Journal of Medical Virology·Peer B ChristensenJørgen Georgsen

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Citations

Jan 17, 2012·AIDS Research and Human Retroviruses·Viviane K Alves-SousaCelso F H Granato
Nov 30, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Myriam VilascoEliane F Meurs
Nov 12, 2009·International Journal of Cancer. Journal International Du Cancer·Mel KrajdenJohn J Spinelli
Mar 1, 2012·Revista Do Instituto De Medicina Tropical De São Paulo·Synara Araújo SilvaJosé Eduardo Levi
Mar 11, 2021·Archives of Virology·Isabel Inês M de Pina-AraujoCaroline Cordeiro Soares

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