Nov 23, 2016

Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing

Methods in Molecular Biology
Miguel Paulo MachadoJoão André Carriço

Abstract

High-Throughput Sequencing (HTS) technologies transformed the microbial typing and molecular epidemiology field by providing the cost-effective ability for researchers to probe draft genomes, not only for epidemiological markers but also for antibiotic resistance and virulence determinants. In this chapter, we provide protocols for the analysis of HTS data for the determination of multilocus sequence typing (MLST) information and for determining presence or absence of antibiotic resistance genes.

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Mentioned in this Paper

Biological Markers
Antibiotic Resistance, Microbial
Molecular Epidemiology
Genome
Genes
Determination Aspects
Research Personnel
Surveillance, Epidemiology, and End Results (Seer) Program
Multilocus Sequence Typing
Computer Programs and Programming

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Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.