Epidemiological Survey of Rotaviruses Responsible for Infantile Diarrhea by the Immunomolecular Technique in Cotonou (Benin, West Africa)

International Journal of Microbiology
Jijoho Mischaël Michel AgblaHonoré Bankolé

Abstract

Rotavirus remains the main causative agent of gastroenteritis in young children, in countries that have not yet introduced the vaccine. Benin, in order to implement the WHO recommendations, projects to introduce the rotavirus vaccine in 2018 as part of its Expanded Program on Immunization. But before the introduction of this vaccine, epidemiological data on rotavirus infections and rotavirus genotypes circulating in Benin should be available. The aim of this study is to generate epidemiological data on infantile rotavirus diarrhea in Benin. In order to determine the epidemiological characteristics and electrophoretypes of rotavirus responsible for gastroenteritis in diarrheic children aged 0 to 5 years, 186 stool samples were collected according to the WHO Rotavirus Laboratory Manual from March 2014 to February 2015 at Suru-Lere University Hospital Center. Detection of rotavirus antigen was performed by the ELISA test, followed by molecular characterization using polyacrylamide gel electrophoresis. 186 stool samples were analyzed for rotavirus, and seventy-three (39.2%) were found to be positive for rotavirus antigen by ELISA. Children aged 3 to 24 months were the most affected by rotavirus diarrhea in this study. Of the sevent...Continue Reading

References

Dec 1, 1987·Journal of Clinical Microbiology·A D Steele, J J Alexander
Jun 1, 1995·Journal of Clinical Microbiology·A D SteeleM J Mphahlele
May 1, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Baoming JiangRoger I Glass
May 15, 2002·The Pediatric Infectious Disease Journal·Mary Allen StaatDavid O Matson
Jul 10, 2003·Journal of Tropical Pediatrics·Mathew D EsonaA Duncan Steele
Sep 13, 2008·Journal of Tropical Pediatrics·M S OdimayoBoaz Adegboro
May 15, 2012·Lancet·Li LiuUNKNOWN Child Health Epidemiology Reference Group of WHO and UNICEF
Jun 30, 2012·The Lancet Infectious Diseases·Manish M PatelUmesh D Parashar
Jan 1, 2013·The Pan African Medical Journal·Magzoub Abbas MagzoubOmran Fadl Osman
Mar 23, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Sonia Etenna Lekana-DoukiEric M Leroy
Jun 13, 2015·International Journal of Microbiology·Paul Erhunmwunse Imade, Nosakhare Odeh Eghafona
Apr 10, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jacqueline E TateUNKNOWN World Health Organization–Coordinated Global Rotavirus Surveillance Network
Apr 22, 2016·The Nigerian Postgraduate Medical Journal·Ezeonwu Bertilla UzomaOguonu Tagbo

❮ Previous
Next ❯

Citations

May 8, 2021·BMC Infectious Diseases·Nosheen BasharatJadoon Khan
May 20, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Tamègnon Victorien DougnonTossou Jacques Dougnon

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
electrophoretic
electrophoresis
electrophoretic profiles

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.