Epidemiology and clinical features of intestinal protozoan infections detected by Real-time PCR in non-native children within an Italian tertiary care children's hospital: A cross-sectional study.

Travel Medicine and Infectious Disease
Elisabetta VenturiniLuisa Galli

Abstract

Enteric parasite infections are underestimated due to the limited sensitivity and specificity of microscopy, which remains the diagnostic gold standard in routine clinical practice. This could be a major problem in high-income countries, where the burden of parasitic diseases is low. In recent years, Multiplex Real-Time polymerase chain reaction (RT-PCR) based methods have been implemented. Therefore, the aim of this study was to evaluate the prevalence of four enteric protozoan species detected by RT-PCR in non-native children in Italy, and to describe their clinical characteristics. Adopted and immigrant children, evaluated for migration health assessment between 2017 and 2020 in a tertiary care children's hospital in Italy, were enrolled. Molecular analysis for Giardia lamblia, Dientamoeba fragilis, Blastocystis hominis, and Entamoeba histolytica, was conducted by in-house RT-PCR. Overall, 209 children were enrolled and 70% of them resulted positive by RT-PCR for at least one enteric parasite. B. hominis (47.8%) was the most commonly identified protozoa, followed by D. fragilis (44.5%). Co-infections with multiple pathogens were detected in 35.4% of the samples. Almost 80% of parasite-positive children were asymptomatic and ...Continue Reading

References

Feb 8, 2003·Journal of Clinical Microbiology·G J J van DoornumH G M Niesters
Apr 18, 2003·The New England Journal of Medicine·Rashidul HaqueWilliam A Petri
Jan 16, 2004·Gut·A C Casburn-Jones, M J G Farthing
Jun 7, 2008·The Journal of Pediatrics·Anita J FuglestadMichael K Georgieff
May 22, 2009·Gastroenterology·Sean W PawlowskiRichard Guerrant
Oct 18, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L MaasB E van Ewijk
May 9, 2014·Journal of Clinical Microbiology·D StarkJ T Ellis
Jun 3, 2014·BMC Infectious Diseases·Adriana CalderaroMaria Cristina Arcangeletti
Apr 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L van Lieshout, M Roestenberg
Jul 6, 2016·Journal of Travel Medicine·Lorenzo ZammarchiAlessandro Bartoloni
Mar 24, 2017·Journal of Clinical Microbiology·Pikka JokelainenChristen R Stensvold
Aug 22, 2017·Frontiers in Cellular and Infection Microbiology·Sonia BoughattasMarawan A Abu-Madi
Sep 8, 2017·Parasites & Vectors·Marawan Abu-MadiAhmed Ismail
Nov 23, 2017·Frontiers in Microbiology·Fabio FormentiZeno Bisoffi
Oct 3, 2018·Enfermedades infecciosas y microbiología clínica·Candela MenéndezAzucena Rodríguez-Guardado
Oct 18, 2018·The American Journal of Tropical Medicine and Hygiene·Lucía MiguelIsrael Molina
Oct 30, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Maria Dullaert- de BoerLesla E S Bruijnesteijn van Coppenraet
Oct 31, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Lucia RehDavid Carmena
May 18, 2020·BMC Infectious Diseases·Sandrine Lydie Oyegue-LiabaguiJean Bernard Lekana-Douki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Journal of Clinical Microbiology
Pikka JokelainenChristen R Stensvold
American Journal of Diseases of Children
M J SpencerM R Chapin
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
D StarkJohn T Ellis
© 2021 Meta ULC. All rights reserved