Epidemiology and molecular characterization of multidrug-resistant Escherichia coli isolates harboring blaCTX-M group 1 extended-spectrum β-lactamases causing bacteremia and urinary tract infection in Manhiça, Mozambique

Infection and Drug Resistance
Elisabet GuiralInacio Mandomando

Abstract

The emergence and spread of extended-spectrum β-lactamases (ESBLs), especially CTX-M, is an important public health problem with serious implications for low-income countries where second-line treatment is often unavailable. Knowledge of the local prevalence of ESBL is critical to define appropriate empirical therapeutic strategies for multidrug-resistant (MDR) organisms. This study aimed to assess and characterize the presence of ESBL and especially CTX-M-producing Escherichia coli MDR isolates from patients with urinary tract infections (UTIs) and bacteremia in a rural hospital in Mozambique. One hundred and fifty-one E. coli isolates from bacteremia and UTI in children were screened for CTX-M, TEM, SHV and OXA β-lactamases by polymerase chain reaction and sequencing. Isolates carrying CTX-M group 1 β-lactamases were further studied. The resistance to other antibiotic families was determined by phenotypic and genotypic methods, the location of the blaCTX-M gene and the epidemiology of the isolates were studied, and extensive plasmid characterization was performed. Approximately 11% (17/151) of E. coli isolates causing bacteremia and UTI were ESBL producers. CTX-M-15 was the most frequently detected ESBL, accounting for 75% of...Continue Reading

Citations

Apr 25, 2019·The American Journal of Tropical Medicine and Hygiene·Anika LarsonDaniel Mäusezahl
Aug 16, 2019·Clinical Microbiology Reviews·Joaquim Ruiz
Dec 11, 2020·BMC Veterinary Research·Anat Shnaiderman-TorbanAmir Steinman
May 20, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Jeremiah SeniRebekah DeVinney
Jun 3, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·José João SumbanaBianca Paglietti
Aug 28, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maria Angeles OrmeñoMaria J Pons
Dec 18, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Masahiro SuzukiYoshichika Arakawa

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Datasets Mentioned

BETA
FN813246.1

Methods Mentioned

BETA
environmental pressure
PCR
electrophoresis
PCRs

Software Mentioned

InfoQuest FP

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