Epidemiology of pertussis in Casablanca (Morocco): contribution of conventional and molecular diagnosis tools

BMC Infectious Diseases
Khalid KatfyNaima Elmdaghri

Abstract

Pertussis, a vaccine preventable disease, is still responsible of significant morbidity and mortality around the world, mostly in newborns. The aim of the present study was (1) to introduce pertussis surveillance in the major pediatric hospital of Casablanca (2) to analyze the prevalence of pertussis among children under 14 years of age and their entourage in Casablanca, Morocco. This is a prospective and non-case controlled study, including children suspected of Pertussis admitted at the Abderrahim Harouchi Pediatric Hospital in Casablanca, from January 2013 to June 2015. Nasopharyngeal samples were obtained for Bordetella spp. culture and Real time PCR detection (RT-PCR) with specific primers of Bordetella spp., B. pertussis, B. parapertussis and B. holmesii. The detection of Bordetella spp. was also performed in some household contacts of the children suspected of pertussis. During the 2.5-years period, a total of 282 samples were collected from hospitalized children (156) and in some of their contacts (126). Among 156 samples from the children (from whom 57% were under 2 month of age), Bordetella DNA was detected in 61% (96/156) by RT-PCR. Among these positive samples, 91.7% (88/96) corresponded to B. pertussis DNA. Further...Continue Reading

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Citations

Jul 6, 2019·Infectious Diseases·Adil RabiNabila Soraa
Jan 16, 2020·BMC Infectious Diseases·Khalid KatfyNaima Elmdaghri
Feb 11, 2021·Klinicheskaia laboratornaia diagnostika·A S PimenovaV A Aleshkin
Apr 2, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Solomon TayeFeleke Moges
Jun 20, 2021·BMC Infectious Diseases·Gaelle NoelFabien Taieb
Apr 5, 2020·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Wujun JiangJun Xu

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Methods Mentioned

BETA
nucleic acid amplification
PCR

Software Mentioned

Excel

Related Concepts