PMID: 6405794May 16, 1983Paper

Epidermal arachidonate lipoxygenase

Biochimica Et Biophysica Acta
T RuzickaM P Printz

Abstract

Guinea pig skin was found to display a high lipoxygenase activity, evidenced by the formation of a hydroxyeicosatetraenoic acid (HETE) from exogenous [14C]arachidonic acid. The lipoxygenase activity was localized to the epidermal layer of the skin, was completely inhibited by eicosatetraynoic acid (ETYA) and slightly enhanced by indomethacin. Susceptibility to inactivation by sulfhydryl-directed reagents indicated that an essential sulfhydryl is present in a hydrophobic region of the molecule. The enzyme exhibited a broad pH activity optimum and a Km of 2.48 . 10(-5) M. The cytosolic enzyme has been partly purified by ammonium sulfate precipitation and two steps of of column chromatography and exhibited an apparent high molecular weight. The lipoxygenase and hydroperoxidase activities were resolvable from one another. The physiological and pathophysiological roles of the enzyme remain to be elucidated.

References

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Citations

Jan 1, 1988·Archives of Dermatological Research·E TragniC L Galli
Jan 13, 1987·Biochimica Et Biophysica Acta·P W Wertz, D T Downing
Apr 3, 1989·Biochimica Et Biophysica Acta·P W Wertz, D T Downing
Oct 1, 1992·The International Journal of Biochemistry·S H Greenberg-LevyS Grossman
Mar 1, 1986·Prostaglandins, Leukotrienes, and Medicine·T NakadateR Kato
Nov 1, 1990·Journal of Dermatological Science·T Ruzicka
Sep 1, 1990·Journal of Dermatological Science·G M RiegerT Krieg
Aug 1, 1996·The International Journal of Biochemistry & Cell Biology·R Bar-NatanS Grossman
Dec 5, 2000·Prostaglandins & Other Lipid Mediators·L Iversen, K Kragballe
Jul 1, 1992·Experimental Dermatology·J M Schröder
Oct 21, 1996·FEBS Letters·E BreitbartS Grossman
Sep 20, 1996·The Journal of Biological Chemistry·C D FunkA R Brash
Apr 1, 1992·European Journal of Clinical Investigation·P ArenbergerT Ruzicka
Jul 1, 1990·The British Journal of Nutrition·J PhoenixM J Jackson
Apr 6, 1995·Biochimica Et Biophysica Acta·L LomnitskiS Grossman
Jan 3, 1986·Biochimica Et Biophysica Acta·J B GermanJ E Kinsella
Aug 6, 1986·Biochimica Et Biophysica Acta·S FlatmanT F Slater
Sep 4, 1987·Biochimica Et Biophysica Acta·D H Nugteren, G A Kivits
Jan 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·F Grimminger, P Mayser
Jul 1, 1985·The Journal of Investigative Dermatology·H KondohH Kanoh
Apr 14, 1986·Biochemical and Biophysical Research Communications·P M Woollard

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