PMID: 42214Oct 1, 1979

Epidermal DNA synthesis in organ culture explants. A study of hairless mouse ear epidermis

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
I L HansteenS B Refsum

Abstract

Explants of split mouse ear were incubated in organ culture for up to 48 h, and the cell proliferation was studied by the addition of Thymidine-methyl-3-H (3HTdR) to the medium during different time periods, mainly for the first 14 h of incubation. Cultures were started at 0900, 2130 and 2300. In all cases the labelling index remained stable for 6-8 h, and then increased. The mean grain count, however, was falling and so was the epidermal DNA-specific uptake of 3HTdR. Based on the experimental results, calculations can be made of the flux of cells through S. It is concluded that the increasing LI is not due to inherent diurnal variation in cell proliferation, and is not a sign of real growth but caused instead by a complete block of the cell exit from S, probably combined with periods of an increased entrance rate into S. Other methodological factors, however, may also contribute to the increasing LI. Hence, this system is not suited for the measurement of factors that influence epidermal DNA synthesis.

References

Jan 1, 1981·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G Fluge, L Aksnes
Jan 1, 1980·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·D A Luke
Dec 1, 1980·The British Journal of Dermatology·R Marks
Jan 1, 1981·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G Fluge, L Aksnes
Mar 1, 1983·The Journal of Pathology·R D van der GaagJ Stam

Citations

Jan 1, 1975·The Journal of Investigative Dermatology·J M YoungS L Cordell
Jan 1, 1977·Archives of Oral Biology·I C Mackenzie, R L Ettinger
Dec 1, 1978·Archives of Dermatological Research·P R Gradwohl
May 1, 1976·The Journal of Investigative Dermatology·K S Stenn, J O Stenn
Jan 1, 1971·Journal of Periodontal Research·R N Powell
May 1, 1972·The British Journal of Dermatology·E Hell, H Maibach
Dec 1, 1960·Experimental Cell Research·S GELFANT

Related Concepts

Culture Techniques
Cell Division Phases
Mice, Nude
Epidermis
DNA, Double-Stranded

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.