Jan 1, 1994

Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines

British Journal of Cancer
S S PrimeC Scully

Abstract

This study examined the expression of epidermal growth factor (EGF) cell-surface receptors, the response to exogenous ligand and the autocrine production of transforming growth factor alpha (TGF-alpha) in normal and carcinoma-derived human oral keratinocytes. One of eight malignant cell lines overexpressed EGF receptors, while the remainder expressed receptor numbers similar to normal cells. Exogenous EGF stimulated incorporation of tritiated thymidine in a dose-dependent manner. In keratinocytes expressing normal numbers of EGF receptors, the cellular response to exogenous EGF correlated positively with total EGF receptor number. SCC-derived keratinocytes produced more TGF-alpha than normal cells. There was no statistical correlation between the autocrine production of TGF-alpha, EGF cell-surface receptor expression and cellular response to exogenous EGF. While the growth-stimulatory effects of exogenous TGF-alpha were inhibited by the addition of a neutralising antibody, the presence of this antibody in conditioned medium failed to produce a similar decrease in growth. The results indicate that overexpression of EGF receptors is not an invariable characteristic of human oral squamous carcinoma-derived cell lines. Further, the...Continue Reading

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Mentioned in this Paper

Epidermal Growth Factor
Thymidine
Transforming Growth Factor Alpha Activity
Keratinocyte
Antibodies, Neutralizing
Immunocytochemistry
Receptor Expression
Tumor Cells, Malignant
Epidermal Growth Factor Receptor
Squamous Cell Carcinoma

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