PMID: 9191980Feb 1, 1997Paper

Epidermal growth factor and transforming growth factor alpha mRNA expression in human breast cancer biopsies; analysis in relation to estradiol, progesterone and EGF receptor content

The Journal of Steroid Biochemistry and Molecular Biology
N ArtagaveytiaS Saez

Abstract

Among the peptide growth factors active in breast glandular cell proliferation epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha) are thought to play a major role in tumour development. They operate through binding to and activation of a common membrane receptor, defined as EGF-R. Their production is modulated by hormones and local growth factors. After it was shown by previous investigation in this laboratory that EGF-R could be detected in 90% of the tumours, but was masked by endogenous ligand in 36% of them, the question was raised as to the level of the ligand's expression in tumour tissue biopsies. Therefore, we investigated the expression of EGF and TGF alpha mRNA in 146 breast cancer biopsies by slot blot analysis using specific 32P-labelled probes. The data were correlated with sex steroids and EGF receptor content. Our results showed that EGF and TGF alpha coexisted in all tumour samples, and that their level of mRNA expression was similar in half of the tumours. Northern blot and polymerase chain reaction (PCR) analysis validated these findings. A significant direct correlation was found between the level of TGF alpha/EGF mRNA expression and the ER/progesterone receptor (PGR) content. TGF ...Continue Reading

References

Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·R B DicksonM E Lippman
Mar 1, 1985·The British Journal of Surgery·J R SainsburyG V Sherbet
Jun 11, 1988·British Medical Journal·M T TraversR C Coombes
Sep 1, 1987·Journal of Cellular Biochemistry·M E LippmanA Kasid
Jan 1, 1984·Breast Cancer Research and Treatment·R PérezA Lage
Nov 1, 1980·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Oct 9, 1980·The New England Journal of Medicine·M B Sporn, G J Todaro

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Citations

May 12, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Felisbina L QueirogaJuan C Illera
Jul 22, 1998·Molecular and Cellular Biology·O W PrallR L Sutherland
Oct 9, 2002·The American Journal of Pathology·Teresa A Rose-HellekantEric P Sandgren

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