Epidermal growth factor can be used in lieu of follicle-stimulating hormone for nuclear maturation of porcine oocytes in vitro

Theriogenology
S J UhmHoon Taek Lee

Abstract

Epidermal growth factor (EGF) has been considered a potential regulator of meiotic and cytoplasmic maturation in mammalian oocytes, but inconsistencies exist between earlier studies, probably due to differences in the culture conditions used. Using a serum- and hormone-free in vitro maturation (IVM) medium, this study investigated the specific contribution of EGF on IVM of porcine (Sus scrofa) oocytes and its interactive effects with follicle-stimulating hormone (FSH), porcine follicular fluid (pFF), cumulus cells, and serum. It was noteworthy that EGF functionally mimicked the action of FSH and could completely replace FSH for nuclear maturation (83.2+/-4.4% vs. 55.9+/-5.2%; mean+/-SEM), whereas EGF had a synergistic effect with FSH on cytoplasmic maturation of porcine oocytes (P<0.05). Specific inhibition of EGF receptor (EGFR) by tyrphostin AG 1478 inhibited both EGF- and FSH-induced meiotic resumption (17.9+/-5.2% and 18.2+/-4.4%, respectively), thereby suggesting that EGFR signaling pathway was essential for oocyte reentry into the meiotic cell cycle. Furthermore, it is possible that FSH action occurs via the EGFR signaling pathway to induce meiotic maturation, although alternate pathways could not be excluded. There were ...Continue Reading

References

Oct 1, 1992·Molecular and Cellular Endocrinology·D Ben-YosefR Shalgi
Aug 31, 1987·Biochemical and Biophysical Research Communications·C J HsuJ M Hammond
Sep 1, 1994·Molecular Reproduction and Development·J Ding, G R Foxcroft
Jun 1, 1994·Molecular Reproduction and Development·S Coskun, Y C Lin
Jan 1, 1996·Reproduction, Fertility, and Development·J J Eppig
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 4, 2002·Molecular and Cellular Endocrinology·Anne Grete ByskovLise Leonardsen
Nov 26, 2002·Endocrinology·Toshio SekiguchiKaoru Miyamoto
May 6, 2003·Reproduction, Fertility, and Development·M PantaleonP L Kaye
Nov 25, 2003·Molecular Endocrinology·Stephen R Hammes
Mar 3, 2004·Molecular Reproduction and Development·Laurence GallClaire Boulesteix
Oct 6, 2004·Zygote : the Biology of Gametes and Early Embryos·Takahiro OyamadaYutaka Fukui
Apr 13, 2005·Theriogenology·Barbara MerloStefano Belluzzi
Apr 29, 2005·Molecular Reproduction and Development·Laurence GallGuy Germain
Jun 30, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Pavneesh MadanAndrew J Watson
Sep 26, 2006·Molecular Reproduction and Development·Mukesh Kumar GuptaHoon Taek Lee
Mar 8, 2007·Molecular Reproduction and Development·Sang Jun UhmHoon Taek Lee
Jun 6, 2007·Molecular Reproduction and Development·Stephen M Downs, Jing Chen
May 1, 2009·Proteomics·Mukesh Kumar GuptaHoon Taek Lee

❮ Previous
Next ❯

Citations

May 19, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Rebecca J HobbsPierre Comizzoli
Mar 20, 2013·Zygote : the Biology of Gametes and Early Embryos·Ziban Chandra DasHoon Taek Lee
May 1, 2012·Reproductive Biology and Endocrinology : RB&E·Ana Marcia M CotaJosé G Franco
Feb 8, 2016·Journal of Assisted Reproduction and Genetics·S LeddaD Bebbere
Jun 16, 2012·Animal Reproduction Science·Wei ZhangXu Zhou
Feb 11, 2016·The Journal of Reproduction and Development·Jin-Woo KimDeog-Bon Koo
Sep 15, 2016·Frontiers in Physiology·Isabella Ellinger
Nov 7, 2016·Biochemical and Biophysical Research Communications·Maria Grazia PalmeriniGuido Macchiarelli
Nov 20, 2016·Molecular Reproduction and Development·Yeo-Jin SonSe-Pill Park
Jul 3, 2013·Cellular Reprogramming·Mukesh Kumar GuptaSang Jun Uhm
May 26, 2017·Reproduction in Domestic Animals = Zuchthygiene·M A GilE A Martinez
May 26, 2018·Journal of Animal Science and Biotechnology·Yitong YuanZhengxing Lian
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Ranjha KhanQinghua Shi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.