PMID: 6980418Apr 1, 1982Paper

Epidermal growth factor carrier protein binds to cells via a complex with released carried protein nexin

Proceedings of the National Academy of Sciences of the United States of America
D J Knauer, D D Cunningham

Abstract

Epidermal growth factor carrier protein (CP) is an arginine endopeptidase bound to epidermal growth factor (EGF) in vivo that processes pro-EGF to EGF and potentiates EGF action. Here, we provide a base for studying the biological functions of CP by showing that highly purified 125I-labeled CP, free of contaminating EGF, is specifically bound and internalized by normal human fibroblasts in serum-free medium. The characteristics of the binding reaction, however, were unusual and not consistent with direct interaction of CP with cell surface receptors. Subsequent experiments showed that cellular binding of 125I-labeled CP was mediated via a cell-secreted protein. We named the protein carrier protein nexin (CPN) because of its close functional similarity to protease nexin, which mediates cellular binding of thrombin or urokinase. Both CPN and protease nexin are secreted by cells, form covalent complexes with regulatory proteases in the extracellular environment, and mediate cellular binding of these proteases, apparently via a cell surface receptor for the nexin moiety of the complex. By several criteria, however, CPN and protease nexin are unique entities. This finding of a specific interaction of a growth factor carrier protein ...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P FreyE M Shooter
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K J Lembach
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J M TaylorW M Mitchell
Sep 1, 1972·The Journal of Experimental Zoology·R A Wallace, T Ho
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L M Hjelmeland
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A LowD D Cunningham

❮ Previous
Next ❯

Citations

Apr 1, 1994·Journal of Cellular Physiology·B W FestoffJ Chao
Jan 1, 1992·Pharmacology & Therapeutics·A I BushC L Masters
Jan 11, 1991·Brain Research. Brain Research Reviews·D C GajdusekK K Hsiao
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J L GrossM D Lane
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L GahringR Daynes
Dec 1, 1985·The Journal of Experimental Medicine·E Remold-O'Donnell
Dec 31, 1992·Annals of the New York Academy of Sciences·W E Van NostrandS L Wagner
Jan 1, 1994·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·G J HoB W Festoff
Feb 28, 1991·Biochemical and Biophysical Research Communications·W E Van NostrandD D Cunningham
Nov 1, 2015·Biochimica Et Biophysica Acta·William E Van Nostrand
Dec 1, 1983·Journal of Cellular Physiology·D J Knauer, D D Cunningham
Jan 1, 1991·Annals of the New York Academy of Sciences·K BeyreutherW Schubert
Jan 1, 1991·Annals of the New York Academy of Sciences·W E Van NostrandD D Cunningham
Jan 1, 1986·Journal of Cellular Biochemistry·D D CunninghamC H Campbell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.