Epidermal growth factor chronically upregulates Ca(2+)-dependent Cl(-) conductance and TMEM16A expression in intestinal epithelial cells.

The Journal of Physiology
Magdalena S Mroz, Stephen J Keely

Abstract

Dysregulated epithelial fluid and electrolyte transport is a common feature of many intestinal disorders. However, molecular mechanisms that regulate epithelial transport processes are still poorly understood, thereby limiting development of new therapeutics. Previously, we showed that epidermal growth factor (EGF) chronically enhances intestinal epithelial secretory function. Here, we investigated a potential role for altered expression or activity of apical Cl(−) channels in mediating the effects of EGF. Cl(−) secretion across monolayers of T(84) colonic epithelia was measured as changes in short-circuit current. Protein expression/phosphorylation was measured by RT-PCR and Western blotting. Under conditions that specifically isolate apical Ca(2+)-activated Cl(−) channel (CaCC) currents, EGF pretreatment (100 ng ml(−1) for 15 min) potentiated carbachol (CCh)-induced responses to 173 ± 25% of those in control cells, when measured 24 h later (n = 26; P < 0.01). EGF-induced increases in CaCC currents were abolished by the transmembrane protein 16A (TMEM16A) inhibitor, T16A(inh)-A01 (10 μm). Furthermore, TMEM16A mRNA and protein expression was increased by EGF to 256 ± 38% (n = 7; P < 0.01) and 297 ± 46% (n = 9, P < 0.001) of con...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J A WagnerP Gardner
Dec 1, 1991·The American Journal of Physiology·A B VaandragerH R De Jonge
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M P Anderson, M J Welsh
Jan 1, 1992·The American Journal of Physiology·U KachintornK Dharmsathaphorn
Jul 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H M BerschneiderD W Powell
Sep 1, 1983·The Journal of General Physiology·K L Kirk, D C Dawson
Feb 28, 1994·Biochemical and Biophysical Research Communications·M GschwendtF Marks
Mar 1, 1996·Gut·R J Playford, N A Wright
Sep 1, 1996·The American Journal of Physiology·J M UribeK E Barrett
Apr 1, 1996·The American Journal of Physiology·Z BorokE D Crandall
Aug 4, 1998·The American Journal of Physiology·D MerlinJ L Madara
Jun 9, 2000·Annual Review of Physiology·K E Barrett, S J Keely
Aug 10, 2000·Biochimica Et Biophysica Acta·T W EcayT D Conner
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·C U NielsenB Brodin
Nov 15, 2002·Canadian Journal of Physiology and Pharmacology·B M ChungD G Gall
Jan 10, 2003·American Journal of Physiology. Cell Physiology·Kenneth R HallowsJ Kevin Foskett
Feb 25, 2003·The Journal of Physiology·Rebecca S LamMarek Duszyk
Jul 31, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gennett M MyhreLaurence J Egan
Jul 8, 2005·American Journal of Physiology. Cell Physiology·Isabel Calvo Del CastilloJeffrey B Matthews
Oct 26, 2005·The Journal of Membrane Biology·G SchultheissM Diener
Apr 1, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·James R BroughmanAndrew P Morris
Sep 28, 2007·American Journal of Physiology. Cell Physiology·Jainuch KanchanapooMrinalini C Rao
Jan 25, 2008·The Journal of Membrane Biology·B HennigM Diener
Apr 11, 2008·American Journal of Physiology. Cell Physiology·Fiona O'MahonyStephen J Keely
Sep 23, 2008·Cell·Björn Christian SchroederLily Yeh Jan
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Kurt D MeyerLubo Zhang
Apr 2, 2009·Journal of Gastroenterology and Hepatology·Virginia Knight, William Sievert
Jun 19, 2009·American Journal of Physiology. Cell Physiology·Biguang TuoHui Dong
Aug 15, 2009·The Journal of Biological Chemistry·Jiraporn OusingsawatKarl Kunzelmann
Dec 17, 2009·Biophysical Journal·Luis J V Galietta
Feb 24, 2010·The Journal of Biological Chemistry·Victor G RomanenkoJames E Melvin
Mar 6, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·M Raschid HodaKim E Barrett
Apr 9, 2010·American Journal of Physiology. Cell Physiology·Hua XuFayez K Ghishan
Jun 29, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wan NamkungA S Verkman
Nov 12, 2010·American Journal of Physiology. Cell Physiology·Chang LauKathryn A DeFea

❮ Previous
Next ❯

Citations

Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Adrian BritschgiMohamed Bentires-Alj
Jun 27, 2013·Journal of Biomedical Science·I-Hui YangWei-Chiao Chang
Feb 12, 2014·Seminars in Cell & Developmental Biology·Fenghua Zeng, Raymond C Harris
Jan 27, 2016·Pflügers Archiv : European journal of physiology·Uhtaek Oh, Jooyoung Jung
Jan 23, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Katarzyna A PiechowiczMarc O Anderson
Mar 13, 2016·Journal of Ethnopharmacology·Hyun Jung KimByung Joo Kim
Mar 19, 2014·Cell Calcium·Yongwoo Jang, Uhtaek Oh
Aug 6, 2014·Biochemical and Biophysical Research Communications·Hai-Yan SongJin-Xia Zhu
Mar 25, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Peter R StregeGianrico Farrugia
Feb 27, 2019·British Journal of Pharmacology·Lena LottigMartin Diener
Apr 3, 2014·Physiological Reviews·Nicoletta Pedemonte, Luis J V Galietta
Jul 6, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Siobhán M HendrickBrian J Harvey
Jan 20, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Magdalena S MrozStephen J Keely
Feb 9, 2017·Genes, Chromosomes & Cancer·Sucheta KulkarniUmamaheswar Duvvuri
Jan 24, 2019·Mediators of Inflammation·Sandra Rayego-MateosMarta Ruiz-Ortega
Nov 5, 2019·Frontiers in Physiology·Karl KunzelmannRainer Schreiber
Jun 10, 2020·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Jian JiaoLuo Zhang
Aug 11, 2017·Scientific Reports·R ReillyJ A Coppinger
Apr 15, 2018·Molecular Cancer Therapeutics·Annie MoisanCéline Adessi
May 7, 2021·The Journal of Biological Chemistry·Xu CaoPingbo Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.